Bioengineered post-natal recombinant tooth bud models.
Bioengineered post-natal recombinant tooth bud models.
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DOI：
10.1002/term.1962
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发表时间：
2017-03
影响因子：
3.3
通讯作者：
Yelick PC
中科院分区：
文献类型：
--
作者：
Zhang W;Vázquez B;Yelick PC
这项研究的长期目标是设计可靠的方法来再生人类的全尺寸和全功能的生物牙齿。在这项研究中，三维（3D）组织工程方法被用来表征完整的出生后牙组织重组结构，和牙细胞悬浮重组结构，作为生物工程牙齿发育的模型。与使用小鼠胚胎牙组织和细胞的研究相反，本文研究了从出生后的猪牙齿和人第三磨牙智齿牙髓中收获的完整牙组织和牙细胞悬液的成牙潜力。将重组三维牙齿构建体在体外成骨培养基中培养1周，然后在无胸腺裸鼠宿主中皮下移植1个月或3个月。随后使用X射线、组织学和免疫组织化学方法的分析表明，大多数重组牙齿结构形成钙化组织，包括骨牙本质、牙本质牙骨质、釉质和由牙本质和釉质组成的形态学上典型的牙冠。从容易获得的出生后牙组织中证明矿化牙组织和牙冠结构的形成是实现建立稳健可靠的人类牙齿再生模型的长期目标的重要一步。
The long-term goal of this study is to devise reliable methods to regenerate full-sized and fully functional biological teeth in humans. In this study, three-dimensional (3D) tissue engineering methods were used to characterize intact postnatal dental tissue recombinant constructs, and dental cell suspension recombinant constructs, as models for bioengineered tooth development. In contrast to studies using mouse embryonic dental tissues and cells, here the odontogenic potential of intact dental tissues and dental cell suspensions harvested from postnatal porcine teeth and human third molar wisdom tooth dental pulp were examined. The recombinant 3D tooth constructs were cultured in osteogenic media in vitro for 1 week before subcutaneous transplantation in athymic nude rat hosts for 1 month or 3 months. Subsequent analyses using X-ray, histological and immunohistochemical methods showed that the majority of the recombinant tooth structures formed calcified tissues, including osteodentin, dentin cementum, enamel and morphologically typical tooth crowns composed of dentin and enamel. The demonstrated formation of mineralized dental tissues and tooth crown structures from easily obtained post-natal dental tissues is an important step toward reaching the long-term goal of establishing robust and reliable models for human tooth regeneration.
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影响因子：
3.7
作者：
Oshima M;Mizuno M;Imamura A;Ogawa M;Yasukawa M;Yamazaki H;Morita R;Ikeda E;Nakao K;Takano-Yamamoto T;Kasugai S;Saito M;Tsuji T
通讯作者：
Tsuji T
大鼠下颌生长的生物工程牙科组织。
影响因子：
7.6
作者：
Duailibi SE;Duailibi MT;Zhang W;Asrican R;Vacanti JP;Yelick PC
通讯作者：
Yelick PC
影响因子：
4.6
作者：
Pöschl, E;Schlötzer-Schrehardt, U;Mayer, U
通讯作者：
Mayer, U
影响因子：
3
作者：
Baba, T;Terashima, T;Sasaki, S
通讯作者：
Sasaki, S
影响因子：
48
作者：
Nakao, Kazuhisa;Morita, Ritsuko;Tsuji, Takashi
通讯作者：
Tsuji, Takashi