Bioengineered dental tissues grown in the rat jaw.
Bioengineered dental tissues grown in the rat jaw.
复制标题
大鼠下颌生长的生物工程牙科组织。
DOI：
10.1177/154405910808700811
复制
发表时间：
2008-08
影响因子：
7.6
通讯作者：
Yelick PC
中科院分区：
文献类型：
--
作者：
Duailibi SE;Duailibi MT;Zhang W;Asrican R;Vacanti JP;Yelick PC
我们的长期目标是开发在颌骨中形成生物工程替代牙齿的方法，这些牙齿表现出与天然牙齿相似的物理特性和功能。我们的研究结果表明，培养的大鼠牙芽细胞，接种到可生物降解的支架上，植入成年大鼠宿主的颌骨中并生长12周，形成了小的，有组织的，生物工程牙冠，含有牙本质，釉质，牙髓和牙周韧带组织，类似于相同的细胞接种支架植入和生长在网膜中。放射学、组织学和免疫组织化学分析表明，生物工程牙齿由组织化的牙本质、釉质和牙髓组织组成。这项研究通过证明生物工程牙齿组织可以在以前丢失的牙齿部位再生，并支持在人类中使用组织工程策略来再生以前丢失和/或缺失的牙齿，从而推进了牙齿组织工程的实际应用。本报告中提出的结果支持生物工程替代牙齿形成的可行性。
Our long-term objective is to develop methods to form, in the jaw, bioengineered replacement teeth that exhibit physical properties and functions similar to those of natural teeth. Our results show that cultured rat tooth bud cells, seeded onto biodegradable scaffolds, implanted into the jaws of adult rat hosts and grown for 12 weeks, formed small, organized, bioengineered tooth crowns, containing dentin, enamel, pulp, and periodontal ligament tissues, similar to identical cell-seeded scaffolds implanted and grown in the omentum. Radiographic, histological, and immunohistochemical analyses showed that bioengineered teeth consisted of organized dentin, enamel, and pulp tissues. This study advances practical applications for dental tissue engineering by demonstrating that bioengineered tooth tissues can be regenerated at the site of previously lost teeth, and supports the use of tissue engineering strategies in humans, to regenerate previously lost and/or missing teeth. The results presented in this report support the feasibility of bioengineered replacement tooth formation in the jaw.
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影响因子：
4.1
作者：
Shi, S;Robey, PG;Gronthos, S
通讯作者：
Gronthos, S
影响因子：
4.2
作者：
Huang, George T-J.;Shagramanova, Kristina;Chan, Selina W.
通讯作者：
Chan, Selina W.
影响因子：
2.7
作者：
Mina, M;Braut, A
通讯作者：
Braut, A
影响因子：
7.6
作者：
Ohazama, A;Modino, SAC;Sharpe, PT
通讯作者：
Sharpe, PT
影响因子：
6.2
作者：
Shi, S;Gronthos, S
通讯作者：
Gronthos, S