FGF-2 combined with bilayer artificial dermis composed of collagen matrix prompts generation of fat pad in subcutis of mice.
FGF-2 combined with bilayer artificial dermis composed of collagen matrix prompts generation of fat pad in subcutis of mice.
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DOI：
10.1007/s00795-018-0203-1
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发表时间：
2019-06
影响因子：
1.8
通讯作者：
Kusumoto K
中科院分区：
文献类型：
--
作者：
Kakudo N;Morimoto N;Ogawa T;Taketani S;Kusumoto K
成纤维细胞生长因子（FGF）-2诱导有丝分裂、血管生成和脂肪生成。本研究探讨了FGF-2与双层人工真皮联合应用对小鼠成脂的诱导作用。FGF-2浸渍的由胶原基质组成的双层人工真皮，PELNAC（Gunze Corp.，Osaka，Japan）皮下植入小鼠的胸部区域。在1、2、3和4周时，收集样品用于H&E染色、血管性血友病因子免疫染色和围脂蛋白免疫染色以检查脂肪组织定位和血管生成。制备未添加FGF-2的胶原基质植入组作为对照。植入FGF-2复合真皮替代物2周后，胶原纤维中出现脂肪细胞。在3-4周时，产生具有新血管形成的脂肪垫。脂肪垫的厚度在2、3和4周时显著增加。随着时间的推移，剩余的胶原蛋白因吸收而减少。对照组未见新的脂肪垫形成。这项研究已经确定了一个有前途的方法，以提高脂肪的影响，在小鼠皮下组织，代表一个潜在的技术，软组织重建。
Fibroblast growth factor (FGF)-2 induces mitogenesis, angiogenesis and adipogenesis. In this study, the adipogenesis-inducing effects of FGF-2 combined with bilayer artificial dermis in mice were evaluated. FGF-2-impregnated bilayer artificial dermis composed of collagen matrix, PELNAC (Gunze Corp., Osaka, Japan) was implanted subcutaneously into the thoracic region of mice. At 1, 2, 3, and 4 weeks, samples were collected for H&E staining, von Willebrand factor immunostaining, and perilipin immunostaining to examine adipose tissue localization and angiogenesis. The collagen matrix-implanted group without the addition of FGF-2 was prepared as a control. At 2 weeks after the implantation of FGF-2 combined with dermal substitutes, adipocytes appeared in the collagen fibers. At 3–4 weeks, a fat pad was generated with neovascularization. The thickness of the fat pad had significantly increased at 2, 3, and 4 weeks. The remaining collagen was decreased by absorption over time. In the control group, no fat pad was newly formed. This study has identified a promising method to enhance adipogenic effects in the murine subcutis, representing a potential technique for soft tissue reconstruction.
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DOI：
10.1054/bjps.2000.3426
发表时间：
2000-12-01
期刊：
BRITISH JOURNAL OF PLASTIC SURGERY
影响因子：
--
作者：
Suzuki, S;Kawai, K;Ikada, Y
通讯作者：
Ikada, Y
DOI：
10.1016/j.bbrc.2007.05.070
发表时间：
2007-07-27
影响因子：
3.1
作者：
Kakudo, Natsuko;Shimotsuma, Ayuko;Kusumoto, Kenji
通讯作者：
Kusumoto, Kenji
影响因子：
2.4
作者：
Mizuno, H;Takeda, A;Uchinuma, E
通讯作者：
Uchinuma, E
DOI：
10.1016/s1357-2725(99)00123-5
发表时间：
2000-02-01
影响因子：
4
作者：
Nugent, MA;Iozzo, RV
通讯作者：
Iozzo, RV
影响因子：
3.7
作者：
Kanazawa S;Fujiwara T;Matsuzaki S;Shingaki K;Taniguchi M;Miyata S;Tohyama M;Sakai Y;Yano K;Hosokawa K;Kubo T
通讯作者：
Kubo T