Enhanced immunosurveillance for animal morbilliviruses using vesicular stomatitis virus (VSV) pseudotypes.
Enhanced immunosurveillance for animal morbilliviruses using vesicular stomatitis virus (VSV) pseudotypes.
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DOI：
10.1016/j.vaccine.2016.10.010
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发表时间：
2016-11-11
期刊：
影响因子：
5.5
通讯作者：
Willett BJ
中科院分区：
文献类型：
--
作者：
Logan N;Dundon WG;Diallo A;Baron MD;James Nyarobi M;Cleaveland S;Keyyu J;Fyumagwa R;Hosie MJ;Willett BJ
病毒特异性中和抗体的测量代表了诊断血清学的“金标准”。对于动物麻疹病毒，如小反刍害虫（PPRV）或牛瘟病毒（RPV），基于活病毒的中和试验需要高水平的生物控制，以防止传染因子意外逃逸。在这项研究中，我们描述了一种基于复制缺陷性水泡性口炎病毒（VSVΔG）的假分型系统的适应性，用于测量动物麻疹病毒的中和抗体。通过在含有荧光素酶标记基因的VSVΔG伪病毒上表达PPRV的血凝素(H)和融合(F)蛋白，可以快速、高灵敏度地测定中和抗体滴度。可以同时测定对四种不同PPRV谱系的血清学反应，并比较对其他麻疹病毒的交叉中和反应。使用这种方法，我们观察到接种减毒PPRV活疫苗诱导的中和抗体滴度低于野生型病毒感染诱导的中和抗体滴度，并且不同疫苗的跨谱系中和水平不同。通过比较感染PPRV或RPV的动物的中和反应，我们发现对同源病毒的反应最高，这表明对血清样本的回顾性分析可用于确认动物接触过的原始病原体的性质。因此，在筛选坦桑尼亚家畜和野生反刍动物的血清时，我们发现了PPRV、犬瘟热病毒（CDV）和与rpv相关的牛麻疹病毒跨物种感染的证据，这表明动物麻疹病毒暴露可能比以前使用现有诊断技术所显示的更为广泛。
The measurement of virus-specific neutralising antibodies represents the “gold-standard” for diagnostic serology. For animal morbilliviruses, such as peste des petits ruminants (PPRV) or rinderpest virus (RPV), live virus-based neutralisation tests require high-level biocontainment to prevent the accidental escape of the infectious agents. In this study, we describe the adaptation of a replication-defective vesicular stomatitis virus (VSVΔG) based pseudotyping system for the measurement of neutralising antibodies against animal morbilliviruses. By expressing the haemagglutinin (H) and fusion (F) proteins of PPRV on VSVΔG pseudotypes bearing a luciferase marker gene, neutralising antibody titres could be measured rapidly and with high sensitivity. Serological responses against the four distinct lineages of PPRV could be measured simultaneously and cross-neutralising responses against other morbilliviruses compared. Using this approach, we observed that titres of neutralising antibodies induced by vaccination with live attenuated PPRV were lower than those induced by wild type virus infection and the level of cross-lineage neutralisation varied between vaccinates. By comparing neutralising responses from animals infected with either PPRV or RPV, we found that responses were highest against the homologous virus, indicating that retrospective analyses of serum samples could be used to confirm the nature of the original pathogen to which an animal had been exposed. Accordingly, when screening sera from domestic livestock and wild ruminants in Tanzania, we detected evidence of cross-species infection with PPRV, canine distemper virus (CDV) and a RPV-related bovine morbillivirus, suggesting that exposure to animal morbilliviruses may be more widespread than indicated previously using existing diagnostic techniques.
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影响因子：
5.5
作者：
Logan N;McMonagle E;Drew AA;Takahashi E;McDonald M;Baron MD;Gilbert M;Cleaveland S;Haydon DT;Hosie MJ;Willett BJ
通讯作者：
Willett BJ
影响因子：
16.6
作者：
通讯作者：
--
影响因子：
5.5
作者：
JONES, L;GIAVEDONI, L;YILMA, T
通讯作者：
YILMA, T
影响因子：
4.4
作者：
Holzer B;Taylor G;Rajko-Nenow P;Hodgson S;Okoth E;Herbert R;Toye P;Baron MD
通讯作者：
Baron MD
影响因子：
3.8
作者：
GRAHAM, FL;SMILEY, J;NAIRN, R
通讯作者：
NAIRN, R