Cloning and characterization of KoOsmotin from mangrove plant Kandelia obovata under cold stress.
Cloning and characterization of KoOsmotin from mangrove plant Kandelia obovata under cold stress.
复制标题
冷胁迫下红树植物秋茄 KoOsmotin 的克隆与表征
DOI：
10.1186/s12870-020-02746-0
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发表时间：
2021-01-06
影响因子：
5.3
通讯作者：
Zheng L
中科院分区：
文献类型：
--
作者：
Fei J;Wang YS;Cheng H;Su YB;Zhong Y;Zheng L
低温是一种主要的非生物压力，它限制了红树林的生产力和分布。
在这项研究中，从红树林K. obovata中克隆了编码渗透素的osmotin（Genbank登录号）的cDNA序列。信号肽，这是大多数渗透蛋白的常见特征，并且与发病机理相关5蛋白质。koosmotin的三维模型包含一个α-螺旋和11β链，由三个特征域形成，koosmotin的比较表现出与渗透素34的phmot the theobroma caCAcienty theybery caCACARET的比较。渗透素/OLP（渗透素样蛋白）。基因表达的质膜和细胞质都表明，k. osmotin在K. obovata的叶片中主要是诱导的，但在茎和根中的绝对性较低。
众所周知，这是探索K. obovata渗透素的第一项研究。
Background
Low temperature is a major abiotic stress that seriously limits mangrove productivity and distribution. Kandelia obovata is the most cold-resistance specie in mangrove plants, but little is known about the molecular mechanism underlying its resistance to cold. Osmotin is a key protein associated with abiotic and biotic stress response in plants but no information about this gene in K. obovata was reported.
Results
In this study, a cDNA sequence encoding osmotin, KoOsmotin (GenBank accession no. KP267758), was cloned from mangrove plant K. obovata. The KoOsmotin protein was composed of 221 amino acids and showed a calculated molecular mass of 24.11 kDa with pI 4.92. The KoOsmotin contained sixteen cysteine residues and an N-terminal signal peptide, which were common signatures to most osmotins and pathogenesis-related 5 proteins. The three-dimensional (3D) model of KoOsmotin, contained one α-helix and eleven β-strands, was formed by three characteristic domains. Database comparisons of the KoOsmotin showed the closest identity (55.75%) with the osmotin 34 from Theobroma cacao. The phylogenetic tree also revealed that the KoOsmotin was clustered in the branch of osmotin/OLP (osmotin-like protien). The KoOsmotin protein was proved to be localized to both the plasma membrane and cytoplasm by the subcellular localization analysis. Gene expression showed that the KoOsmotin was induced primarily and highly in the leaves of K. obovata, but less abundantly in stems and roots. The overexpressing of KoOsmotin conferred cold tolerance in Escherichia coli cells.
Conclusion
As we known, this is the first study to explore the osmotin of K. obovata. Our study provided valuable clues for further exploring the function of KoOsmotin response to stress.
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