Studies of some naturally occurring auxotrophs of Neisseria gonorrhoeae.
Studies of some naturally occurring auxotrophs of Neisseria gonorrhoeae.
复制标题
对一些天然存在的淋病奈瑟菌营养缺陷型的研究。
DOI：
10.1099/00221287-121-1-85
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Heym,GA
中科院分区：
文献类型：
--
作者：
Juni,E;Heym,GA
一株淋球菌需要精氨酸、脯氨酸、谷氨酸和胱氨酸作为营养补充剂，经几步转化，使其在含有胱氨酸作为唯一生长因子的简单矿物培养基中生长，所用的DNA来自该生物体的几种临床分离菌株。利用来自天然存在的营养缺陷型（auxo-types）的DNA，已知需要精氨酸、次黄嘌呤和尿嘧啶（Arg−Hyx−Ura−）以及其他因子，可以将营养标记一次一个地转移到这样的原养型中，以获得七个单一标记营养缺陷型菌株。将三种不同的尿嘧啶标记物、两种不同的次黄嘌呤标记物、精氨酸标记物和异亮氨酸-缬氨酸标记物各自引入单独的菌株中。从114份独立分离的N.淋病，54能够转化所有七个单一标记菌株的原养型。从43个菌株的DNA样品的单一标记菌株未能被转化为原养型，从而表明，所有这些菌株具有至少六个共同的营养病变。两个菌株被证明包含所有七个营养病变，而几个菌株包含一些，但不是所有的七个病变。七个单一标记菌株中的六个已被证明在低频率下自发地恢复到原养型。在原养型菌株的构建过程中，观察到赋予对复合培养基中营养物的生长抑制敏感性的基因偶尔沿着原养型转移。
A strain ofNeisseria gonorrhoeaerequiring arginine, proline, glutamate and cystine as nutritional supplements was transformed, in several steps, to grow in a simple mineral medium containing cystine as the only growth factor with DNA from several clinically isolated strains of this organism. Using DNA from naturally occurring auxotrophs (auxo-types) known to require arginine, hypoxanthine and uracil (Arg−Hyx−Ura−), as well as other factors, it was possible to transfer nutritional markers, one at a time, into such prototrophs to obtain seven single marker auxotrophic strains. Three different uracil markers, two different hypoxanthine markers, an arginine marker, and an isoleucine-valine marker were each introduced into separate strains. Of 114 DNA samples from independently isolated strains ofN. gonorrhoeae, 54 were able to transform all seven single marker strains to prototrophy. Six of the single marker strains failed to be transformed to prototrophy by DNA samples from 43 strains, thus demonstrating that all these strains possess at least six nutritional lesions in common. Two strains were shown to contain all seven nutritional lesions, whereas several strains contained some but not all of the seven lesions. Six of the seven single marker strains have been shown to revert spontaneously to prototrophy at low frequencies. During construction of prototrophic strains it was observed that genes conferring sensitivity to growth inhibition by nutrients in complex media were occasionally transferred along with prototrophy.
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引起播散性淋球菌感染的淋球菌对正常人血清的杀菌作用具有抵抗力。
影响因子：
15.9
作者：
G K Schoolnik;T. M. Buchanan;K K Holmes
通讯作者：
K K Holmes
化学成分确定的培养基中淋病奈瑟菌、脑膜炎奈瑟菌和乳酸奈瑟菌的营养状况以及淋球菌分型的生长要求的使用。
影响因子：
6.4
作者：
B. Catlin
通讯作者：
B. Catlin
影响因子：
9.4
作者：
A. Janik;E. Juni;G. Heym
通讯作者：
G. Heym
通过亚硝酸生产脑膜炎奈瑟菌营养缺陷型突变体。
DOI：
10.1111/apm.1965.63.4.615
发表时间：
1965
期刊：
Acta pathologica et microbiologica Scandinavica
影响因子：
--
作者：
S. Lie
通讯作者：
S. Lie
从脑膜炎奈瑟菌中分离营养缺陷型。
DOI：
10.1111/apm.1965.63.3.435
发表时间：
1965
期刊：
Acta pathologica et microbiologica Scandinavica
影响因子：
--
作者：
K. Jyssum
通讯作者：
K. Jyssum