Gross karyotypic and phenotypic alterations among different progenies of the Candida glabrata CBS138/ATCC2001 reference strain.
Gross karyotypic and phenotypic alterations among different progenies of the Candida glabrata CBS138/ATCC2001 reference strain.
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DOI：
10.1371/journal.pone.0052218
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Weig M
中科院分区：
文献类型：
--
作者：
Bader O;Schwarz A;Kraneveld EA;Tangwattanachuleeporn M;Schmidt P;Jacobsen MD;Gross U;De Groot PW;Weig M
基因组可塑性是许多真菌（包括人类致病酵母光滑念珠菌）适应环境线索（如宿主反应和抗真菌药物压力）的机制。本研究对世界范围内广泛使用的C. glabrata参考菌株CBS 138/ATCC 2001。从不同的科学实验室获得该野生型菌株和遗传修饰后代的一组十个谱系，并分析基因型和表型改变。即使衍生物是无法区分的多位点序列分型，不同的表型组，与特定的核型变化进行了观察。此外，在塑料表面的粘附能力出现的修改，被证明与粘附素基因表达的定量变化，而不是subtelomeric基因的丢失或粘附素基因内的大卫星重复序列的数量的差异。这些结果证实了C. glabrata的研究表明，染色体畸变和功能适应不仅可能发生在感染期间和抗菌治疗下，而且也可能发生在没有极端选择压力的实验室条件下。这些改变可显著影响表型特性，例如细胞表面属性，包括粘附和细胞壁碳水化合物组成，因此，如果未被注意，可能会影响遗传研究的结果。
Genomic plasticity is a mechanism for adaptation to environmental cues such as host responses and antifungal drug pressure in many fungi including the human pathogenic yeast Candida glabrata. In this study we evaluated the phenotypic and genotypic stability of the world-wide used C. glabrata reference strain CBS138/ATCC2001 under laboratory conditions. A set of ten lineages of this wild type strain and genetically modified progenies were obtained from different scientific laboratories, and analyzed for genotypic and phenotypic alterations. Even though the derivates were indistinguishable by multi locus sequence typing, different phenotypic groups that correlated with specific karyotypic changes were observed. In addition, modifications in the adherence capacity to plastic surface emerged that were shown to correlate with quantitative changes in adhesin gene expression rather than subtelomeric gene loss or differences in the number of macrosatellite repeats within adhesin genes. These results confirm the genomic plasticity of C. glabrata and show that chromosomal aberrations and functional adaptations may occur not only during infection and under antimicrobial therapy, but also under laboratory conditions without extreme selective pressures. These alterations can significantly affect phenotypic properties such as cell surface attributes including adhesion and the cell wall carbohydrate composition and therefore, if unnoticed, may adulterate the outcome of genetic studies.
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影响因子：
3.5
作者：
KITADA, K;YAMAGUCHI, E;ARISAWA, M
通讯作者：
ARISAWA, M
影响因子：
64.8
作者：
Dujon, B;Sherman, D;Souciet, JL
通讯作者：
Souciet, JL
影响因子：
--
作者：
de Groot, Piet W. J.;Kraneveld, Eefje A.;Weig, Michael
通讯作者：
Weig, Michael
影响因子：
4.5
作者：
Fischer G;Rocha EP;Brunet F;Vergassola M;Dujon B
通讯作者：
Dujon B
草绿色念珠菌的多焦点序列分型揭示了地理上丰富的支系
影响因子：
9.4
作者：
Dodgson, AR;Pujol, C;Fox, AJ
通讯作者：
Fox, AJ