Combination Effects of Herbs in a Multi-herbal Formula: Expression of Juzen-taiho-to's Immuno-modulatory Activity on the Intestinal Immune System.
Combination Effects of Herbs in a Multi-herbal Formula: Expression of Juzen-taiho-to's Immuno-modulatory Activity on the Intestinal Immune System.
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DOI：
10.1093/ecam/neh004
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发表时间：
2004-06-01
期刊：
影响因子：
--
通讯作者：
Yamada H
中科院分区：
文献类型：
--
作者：
Kiyohara H;Matsumoto T;Yamada H
传统日本（汉方）、中国和韩国药物的草药配方通常在单一配方中包含多种草药。这些药物有望通过多味药的化学、药理和药物组合作用来显示其临床疗效。然而，到目前为止，几乎没有努力科学地澄清这种组合效应的性质。有趣的是，例如，汉方药物Juzen-taiho-to（中国的Shi-Quan-Da-Bu-Tang）虽然刺激Peyer's patch细胞的免疫功能，但其单一成分的草药都没有显示出这种活性。因此，我们研究了在Juzen-taiho-to公式中草药的组合效果，以表达其免疫刺激活性。10味药的复方制剂“十善大宝汤”一般被认为是由两种基本方组成，每种基本方由四种不同的药物和另外两种药物组成。基于这两种基本配方的草药组合评价了它们对体外和离体小鼠派伊尔氏斑细胞产生细胞因子的刺激活性。10味药中的6味合煎对派尔氏斑细胞表达刺激活性至关重要。3D-HPLC分析表明，不同中药煎煮后，其化学成分除了发生数量变化外，还发生了变化。Juzen-taiho-to对Peyer's patch细胞的刺激活性来自其六种主要成分草药的联合作用。这种组合效应是基于组分草药成分之间的物理化学相互作用。
Herbal formulas of traditional Japanese (Kampo), Chinese and Korean medicines usually comprise multiple herbs in a single formula. These medicines are expected to show their clinical effects by chemical, pharmacological and pharmaceutical combination effects of multi-herbs. However, little effort has been made so far to scientifically clarify the nature of such combination effects. Interestingly, for example, though a Kampo medicine Juzen-taiho-to (Shi-Quan-Da-Bu-Tang in Chinese) stimulates the immune functions of Peyer's patch cells, none of its single component herbs shows such activity. We thus examined the combination effect of herbs in the Juzen-taiho-to formula for the expression of its immuno-stimulating activity. Juzen-taiho-to, a composite formula of 10 herbs, has been generally considered to comprise two kinds of basic formula, each of which consists of four different herbs in addition to two others. The combinations of herbs based on these two basic formulas were evaluated for their stimulating activities on cytokine production from murine Peyer's patch cells both in vitro and ex vivo. Combined decoction of six among 10 herbs in Juzen-taiho-to is crucial for the expression of its stimulating activity on Peyer's patch cells. 3D-HPLC analysis of the ingredients in the fractions from the combined decoctions indicated that, in addition to quantitative changes of ingredients, alterations occur in their chemical composition by decoction of different herbs. The stimulating activity of Juzen-taiho-to on Peyer's patch cells results from the combination effect of its six essential component herbs. This combination effect is based on physicochemical interactions among the ingredients of the component herbs.
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DOI：
10.1016/s0162-3109(99)00166-6
发表时间：
2000-02-01
期刊：
IMMUNOPHARMACOLOGY
影响因子：
--
作者：
Matsumoto, T;Sakurai, MH;Yamada, H
通讯作者：
Yamada, H
DOI：
10.1016/0192-0561(94)00056-5
发表时间：
1994-11-01
期刊：
INTERNATIONAL JOURNAL OF IMMUNOPHARMACOLOGY
影响因子：
--
作者：
TAKEMOTO, N;KIYOHARA, H;KAWAMURA, H
通讯作者：
KAWAMURA, H
影响因子：
2
作者：
Onishi, Y;Yamaura, T;Saika, I
通讯作者：
Saika, I
影响因子：
7.9
作者：
Kiyohara, H;Matsumoto, T;Yamada, H
通讯作者：
Yamada, H
影响因子：
2.7
作者：
Kiyohara, H;Matsumoto, T;Yamada, H
通讯作者：
Yamada, H