Peripheral Administration of NMU Promotes White Adipose Tissue Beiging and Improves Glucose Tolerance.
Peripheral Administration of NMU Promotes White Adipose Tissue Beiging and Improves Glucose Tolerance.
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NMU 外周给药促进白色脂肪组织米色化并改善葡萄糖耐量
DOI：
10.1155/2021/6142096
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发表时间：
2021
影响因子：
2.8
通讯作者：
Bi Y
中科院分区：
文献类型：
--
作者：
Yuan Y;Wang H;He J;Sun H;Zhu D;Bi Y
靶向白色脂肪组织（WAT）的脂肪被认为是增加产热和改善葡萄糖代谢的有效途径。神经介肽U（NMU）是一种增加能量消耗的神经肽，但其对WAT平衡和葡萄糖稳态的影响尚待研究。 雄性C57 BL/6小鼠用高脂饮食（HFD）喂养以诱导肥胖和高血糖，然后用慢性皮下注射NMU治疗。每天记录体重和摄食量。注射14天后，进行腹膜内葡萄糖耐量试验和18 F-氟脱氧葡萄糖微型正电子发射断层扫描/计算机断层扫描（18 F-FDG微型PET/CT）扫描。收集皮下WAT（sWAT）和肩胛间棕色脂肪组织，用于评估脂肪细胞大小、解偶联蛋白1（Ucp 1）和其他产热相关基因的表达。提取皮下WAT的基质血管部分用于测量2型先天性淋巴细胞（ILC 2）比例。 葡萄糖耐量显着改善外周给予NMU。Micro-PET/CT显示NMU促进WAT染色，这通过苏木精和伊红（H&E）染色和免疫组织化学进一步证实。在饮食诱导肥胖（DIO）小鼠中，NMU激活WAT中的产热相关基因。此外，NMU刺激WAT的基质血管部分中的ILC 2。 总而言之，我们的研究表明，外周给予NMU是促进WAT beiging和改善糖耐量受损的潜在治疗策略。
Targeting white adipose tissue (WAT) beiging has been proposed as an effective way to increase thermogenesis and improve glucose metabolism. Neuromedin U (NMU) is a neuropeptide that could increase energy expenditure, while its effects on WAT beiging and glucose homeostasis remain to be investigated. Male C57BL/6 mice were fed with high fat diet (HFD) to induce obesity and hyperglycemia and then treated with chronic subcutaneous injection of NMU. Body weight and food intake were recorded daily. After 14 days of injection, intraperitoneal glucose tolerance tests and 18F-fluorodeoxyglucose micro-positron emission tomography/computed tomography (18F-FDG micro-PET/CT) scans were conducted. Subcutaneous WAT (sWAT) and interscapular brown adipose tissue were collected for the evaluation of adipocyte size, expression of uncoupling protein 1 (Ucp1), and other thermogenic-related genes. Stromal vascular fraction of subcutaneous WAT was extracted for the measurement of type 2 innate lymphocytes (ILC2s) proportions. Glucose tolerance was markedly improved by peripherally administered NMU. Micro-PET/CT suggested that NMU promoted WAT beiging, which was further confirmed by haematoxylin and eosin (H&E) staining and immunohistochemistry. In diet-induced-obese (DIO) mice, NMU activated thermogenic-related genes in WAT. In addition, NMU stimulated ILC2s in the stromal vascular fraction of WAT. Taken together, our study indicates that peripheral administration of NMU is a potential therapeutic strategy for the promotion of WAT beiging and the improvement of impaired glucose tolerance.
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影响因子：
16.2
作者：
Bragg F;Tang K;Guo Y;Iona A;Du H;Holmes MV;Bian Z;Kartsonaki C;Chen Y;Yang L;Sun Q;Dong C;Chen J;Collins R;Peto R;Li L;Chen Z;China Kadoorie Biobank (CKB) Collaborative Group
通讯作者：
China Kadoorie Biobank (CKB) Collaborative Group
影响因子：
3.3
作者：
BALLESTA, J;CARLEI, F;POLAK, JM
通讯作者：
POLAK, JM
影响因子：
6.9
作者：
Orava, Janne;Nuutila, Pirjo;Virtanen, Kirsi A.
通讯作者：
Virtanen, Kirsi A.
影响因子：
3.5
作者：
Ingallinella, Paolo;Peier, Andrea M.;Pessi, Antonello
通讯作者：
Pessi, Antonello
DOI：
10.1006/bbrc.2000.3669
发表时间：
2000-10-14
影响因子：
3.1
作者：
Nakazato, M;Hanada, R;Matsukura, S
通讯作者：
Matsukura, S