Heparin/Collagen-REDV Modification of Expanded Polytetrafluoroethylene Improves Regional Anti-thrombosis and Reduces Foreign Body Reactions in Local Tissues.
Heparin/Collagen-REDV Modification of Expanded Polytetrafluoroethylene Improves Regional Anti-thrombosis and Reduces Foreign Body Reactions in Local Tissues.
复制标题
发泡聚四氟乙烯的肝素/胶原-REDV改性可提高局部抗血栓能力并减少局部组织的异物反应
DOI：
10.3389/fbioe.2022.916931
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发表时间：
2022
影响因子：
5.7
通讯作者：
Jia, Bing
中科院分区：
文献类型：
--
作者：
Shan, Yaping;Chen, Gang;Shi, Qiqi;Huang, Jiaxi;Mi, Yaping;Zhang, Wenbo;Zhang, Huifeng;Jia, Bing
关键词：
由于血栓形成和内膜增生，心血管系统中的膨体聚四氟乙烯（ePTFE）假体植入物从长远来看失败率很高。尽管多种表面改性方法已被应用于提高ePTFE的抗血栓和原位内皮化能力，但没有一种方法在体内产生出色的效果。我们前期的研究将肝素/胶原多层膜与REDV肽相结合来修饰ePTFE，体外结果表明肝素/胶原-REDV修饰ePTFE可以提高细胞相容性和抗血小板性能。本研究进一步阐明了物理变化、选择性内皮细胞捕获能力和体内性能。物理测试表明，这种改性提高了ePTFE的亲水性、柔韧性和强度。共培养内皮细胞和血管平滑肌细胞的竞争实验验证了肝素/胶原-REDV修饰对内皮细胞捕获具有高度特异性。构建兔动物模型来评估植入右心室流出道的改性 ePTFE 的体内性能。结果表明，肝素/胶原-REDV修饰安全，可促进内皮化，成功实现局部抗血栓，且不影响全身凝血功能。与改性ePTFE接触的组织的病理表现和mRNA表达模式表明，这种改性方法可能减少M2型巨噬细胞的浸润以及与免疫和炎症反应相关的基因的表达。肝素/胶原-REDV修饰可降低ePTFE植入相关并发症的发生率，具有良好的临床应用前景。
Prosthetic implants of expanded polytetrafluoroethylene (ePTFE) in the cardiovascular system have a high failure rate over the long term because of thrombosis and intimal hyperplasia. Although multiple surface modification methods have been applied to improve the anti-thrombotic and in situ endothelialization abilities of ePTFE, none have delivered outstanding results in vivo. Our previous study combined heparin/collagen multilayers and REDV peptides to modify ePTFE, and the in-vitro results showed that modification ePTFE with heparin/collagen-REDV can promote the cytocompatibility and antiplatelet property. This study illustrated the physical change, selective endothelial cells capture ability, and in vivo performance in further. The physical test demonstrated that this modification improved the hydrophilicity, flexibility and strength of ePTFE. A competition experiment of co-cultured endothelial cells and vascular smooth muscle cells verified that the heparin/collagen-REDV modification had high specificity for endothelial cell capture. A rabbit animal model was constructed to evaluate the in vivo performance of modified ePTFE implanted in the right ventricular outflow tract. The results showed that heparin/collagen-REDV modification was safe, promoted endothelialization, and successfully achieved regional anti-thrombosis without influencing body-wide coagulation function. The pathologic manifestations and mRNA expression pattern in tissues in contact with modified ePTFE indicated that this modification method may reduce M2-type macrophage infiltration and the expression of genes related to immune and inflammatory responses. The heparin/collagen-REDV modification may lower the incidence of complications related to ePTFE implantation and has good prospects for clinical use.
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影响因子：
5.8
作者：
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通讯作者：
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影响因子：
14
作者：
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影响因子：
9.5
作者：
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通讯作者：
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