Establishment and characterization of an embryonic pericyte cell line.
Establishment and characterization of an embryonic pericyte cell line.
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DOI：
10.1111/micc.12461
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发表时间：
2018-07
期刊：
影响因子：
--
通讯作者：
Chappell JC
中科院分区：
文献类型：
--
作者：
Zhao H;Darden J;Chappell JC
周细胞是嵌入基底膜内的特化血管周围细胞。这些细胞包被内皮细胞的近腔表面并促进微血管稳态。最近发现的独特的周细胞功能，特别是在神经组织中，强调需要克服现有的挑战，建立一个功能验证的周细胞系。在这里，我们提出了解决这些挑战的方法，以及用于在体外和离体实验模型的胚胎周细胞系。我们从胚胎第12.5天（E12.5）小鼠中分离出神经胶质抗原-2（NG 2）：DsRed+周细胞的富集群体。将该周细胞系与小鼠胚胎成纤维细胞（MEFs）在基因表达、细胞形态和迁移以及在连接稳定和血管生成过程中与内皮细胞的接合方面进行比较。NG 2+周细胞显示基因表达模式，细胞形态和2D迁移行为不同于MEFs。在三种不同的血管形成模型中，来自这条线的周细胞迁移到发育中的血管中并并入其中。当与人脐静脉内皮细胞（HUVEC）共培养时，与MEF相比，这些周细胞刺激HUVEC之间更稳健的VE-钙粘蛋白连接，并且有助于HUVEC组织成原始血管结构。我们的数据支持在广泛的模型中使用这种周细胞系，以进一步了解正常和病理条件下的周细胞功能。
Pericytes are specialized perivascular cells embedded within the basement membrane. These cells envelope the abluminal surface of endothelial cells and promote microvessel homeostasis. Recent discoveries of unique pericyte functions, particularly in neural tissues, underscore the need for overcoming existing challenges in establishing a functionally validated pericyte cell line. Here, we present methodologies for addressing these challenges as well as an embryonic pericyte cell line for use with in vitro and ex vivo experimental models. We isolated an enriched population of Neural Glial Antigen-2 (NG2):DsRed+ pericytes from embryonic day 12.5 (E12.5) mice. This pericyte cell line was compared to mouse embryonic fibroblasts (MEFs) with respect to gene expression, cell morphology and migration, and engagement with endothelial cells during junction stabilization and angiogenesis. NG2+ pericytes displayed gene expression patterns, cell morphology, and 2D migration behaviors distinct from MEFs. In three different vessel formation models, pericytes from this line migrated to and incorporated into developing vessels. When co-cultured with human umbilical vein endothelial cells (HUVECs), these pericytes stimulated more robust VE-Cadherin junctions between HUVECs as compared to MEFs, as well as contributed to HUVEC organization into primitive vascular structures. Our data support use of this pericyte cell line in a broad range of models to further understand pericyte functionality during normal and pathological conditions.
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影响因子：
14.8
作者：
Levenberg, Shulamit;Ferreira, Lino S.;Chen-Konak, Limor;Kraehenbuehl, Thomas P.;Langer, Robert
通讯作者：
Langer, Robert
影响因子：
4.6
作者：
He L;Vanlandewijck M;Raschperger E;Andaloussi Mäe M;Jung B;Lebouvier T;Ando K;Hofmann J;Keller A;Betsholtz C
通讯作者：
Betsholtz C
DOI：
10.1111/micc.12107
发表时间：
2014-05
期刊：
Microcirculation (New York, N.Y. : 1994)
影响因子：
--
作者：
Kelly-Goss MR;Sweat RS;Stapor PC;Peirce SM;Murfee WL
通讯作者：
Murfee WL
影响因子：
20.3
作者：
Drake, CJ;Fleming, PA
通讯作者：
Fleming, PA
影响因子：
16.2
作者：
Hill, Robert A.;Tong, Lei;Yuan, Peng;Murikinati, Sasidhar;Gupta, Shobhana;Grutzendler, Jaime
通讯作者：
Grutzendler, Jaime