Regional Blood Flow in the Normal and Ischemic Brain Is Controlled by Arteriolar Smooth Muscle Cell Contractility and Not by Capillary Pericytes.
Regional Blood Flow in the Normal and Ischemic Brain Is Controlled by Arteriolar Smooth Muscle Cell Contractility and Not by Capillary Pericytes.
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DOI：
10.1016/j.neuron.2015.06.001
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发表时间：
2015-07-01
期刊：
影响因子：
16.2
通讯作者：
Grutzendler, Jaime
中科院分区：
文献类型：
--
作者：
Hill, Robert A.;Tong, Lei;Yuan, Peng;Murikinati, Sasidhar;Gupta, Shobhana;Grutzendler, Jaime
脑血流量的精确调节对于正常的脑功能至关重要，并且其中断是许多神经病理学的基础。在神经血管耦合过程中，平滑肌覆盖的小动脉或周细胞覆盖的毛细血管控制血管运动的程度仍然存在争议。我们发现，小鼠和人类的毛细血管周细胞不表达平滑肌肌动蛋白，并且在形态和功能上与相邻的前毛细血管平滑肌细胞（SMC）不同。使用光学成像，我们研究了活体小鼠血管树各个部位的血流调节。光遗传，晶须刺激或皮层扩散去极化引起的微血管直径或流量的变化，SMC，但不周细胞覆盖的微血管。在脑缺血的早期阶段，短暂的SMC而不是周细胞收缩是导致血栓形成和远端微血管闭塞的灌注不足的主要原因。因此，毛细血管周细胞不收缩，并且在生理和病理条件下脑血流的调节由小动脉平滑肌细胞介导。
The precise regulation of cerebral blood flow is critical for normal brain function and its disruption underlies many neuropathologies. The extent to which smooth muscle-covered arterioles or pericyte-covered capillaries control vasomotion during neurovascular coupling remains controversial. We found that capillary pericytes in mice and humans do not express smooth muscle actin and are morphologically and functionally distinct from adjacent precapillary smooth muscle cells (SMCs). Using optical imaging we investigated blood flow regulation at various sites on the vascular tree in living mice. Optogenetic, whisker stimulation or cortical spreading depolarization caused microvascular diameter or flow changes in SMC but not pericyte-covered microvessels. During early stages of brain ischemia, transient SMC but not pericyte constrictions were a major cause of hypoperfusion leading to thrombosis and distal microvascular occlusions. Thus, capillary pericytes are not contractile and regulation of cerebral blood flow in physiological and pathological conditions is mediated by arteriolar smooth muscle cells.
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影响因子：
25
作者：
Hill, Robert A.;Patel, Kiran D.;Goncalves, Christopher M.;Grutzendler, Jaime;Nishiyama, Akiko
通讯作者：
Nishiyama, Akiko
DOI：
10.1073/pnas.2133652100
发表时间：
2003-10-28
影响因子：
11.1
作者：
Chaigneau, E;Oheim, M;Charpak, S
通讯作者：
Charpak, S
DOI：
10.1073/pnas.1100428108
发表时间：
2011-05-17
影响因子：
11.1
作者：
Drew, Patrick J.;Shih, Andy Y.;Kleinfeld, David
通讯作者：
Kleinfeld, David
影响因子：
25
作者：
Blinder, Pablo;Tsai, Philbert S.;Kaufhold, John P.;Knutsen, Per M.;Suhl, Harry;Kleinfeld, David
通讯作者：
Kleinfeld, David
影响因子：
1.5
作者：
Armstrong, John J.;Larina, Irina V.;Dickinson, Mary E.;Zimmer, Warren E.;Hirschi, Karen K.
通讯作者：
Hirschi, Karen K.