Proteome changes in the plasma of Pieris rapae parasitized by the endoparasitoid wasp Pteromalus puparum
Proteome changes in the plasma of Pieris rapae parasitized by the endoparasitoid wasp Pteromalus puparum
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内寄生蜂 Pteromalus puparum 寄生的菜青虫血浆中蛋白质组的变化
DOI：
10.1631/jzus.b1000158
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发表时间：
2011-02
影响因子：
5.1
通讯作者：
Cui HU
中科院分区：
文献类型：
--
作者：
Jia-ying ZHU;Qi FANG;Gong-yin YE;Cui HU
内寄生蜂蛹金小蜂的寄生作用引起了淡斑粉蝶血浆蛋白的变化。利用蛋白质组学方法对血浆蛋白进行分析，发现寄生24 h后，7种蛋白质在寄主蛹中差异表达。它们是伪装样丝氨酸蛋白酶同源物（MSPH）、烯醇化酶（Eno）、胆色素结合蛋白（BBP）、成虫盘生长因子（IDGF）、鸟氨酸脱羧酶（ODC）、细胞视黄酸结合蛋白（CRABP）和一个未知功能蛋白。利用cDNA末端快速扩增-聚合酶链反应（RACE-PCR）技术成功克隆了MSPH、Eno和BBP的全长cDNA序列。逆转录-聚合酶链反应（RT-PCR）分析表明，寄生对蛹脂肪体中MSPH和BBP基因的转录水平有诱导作用，其变化与寄生后这些基因的翻译变化一致，而Eno和IDGF基因的转录水平不受寄生的影响。本研究将有助于更好地了解寄生虫诱导宿主改变的分子基础，与先天免疫反应，解毒和能量代谢有关。
Parasitism by the endoparasitoid wasp Pteromalus puparum causes alterations in the plasma proteins of Pieris rapae. Analysis of plasma proteins using a proteomic approach showed that seven proteins were differentially expressed in the host pupae after 24-h parasitism. They were masquerade-like serine proteinase homolog (MSPH), enolase (Eno), bilin-binding protein (BBP), imaginal disc growth factor (IDGF), ornithine decarboxylase (ODC), cellular retinoic acid binding protein (CRABP), and one unknown function protein. The full length cDNA sequences of MSPH, Eno, and BBP were successfully cloned using rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR). Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis indicated that the transcript levels of MSPH and BBP in the fat bodies of host pupae were inducible in response to the parasitism and their variations were consistent with translational changes of these genes after parasitism, while the transcript levels of Eno and IDGF were not affected by parasitism. This study will contribute to the better understanding of the molecular bases of parasitoidinduced host alterations associated with innate immune responses, detoxification, and energy metabolism.
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影响因子：
2.2
作者：
K. Song;M. Jung;Jai-Hoon Eum;I. Hwang;S. Han
通讯作者：
K. Song;M. Jung;Jai-Hoon Eum;I. Hwang;S. Han
影响因子：
3.8
作者：
Jun Zhang;Sachio Iwai;T. Tsugehara;M. Takeda
通讯作者：
Jun Zhang;Sachio Iwai;T. Tsugehara;M. Takeda
影响因子：
2.2
作者：
E. H. Richards;J. P. Edwards
通讯作者：
E. H. Richards;J. P. Edwards
DOI：
--
发表时间：
1998
期刊：
--
影响因子：
--
作者：
P. Brey;D. Hultmark
通讯作者：
P. Brey;D. Hultmark
DOI：
10.1631/jzus.b0820314
发表时间：
2009-05
期刊：
Journal of Zhejiang University SCIENCE B
影响因子：
--
作者：
Jiaying Zhu;G. Yè;Qi Fang;Cui Hu
通讯作者：
Jiaying Zhu;G. Yè;Qi Fang;Cui Hu