Overexpression of the kiwifruit SVP3 gene affects reproductive development and suppresses anthocyanin biosynthesis in petals, but has no effect on vegetative growth, dormancy, or flowering time.
Overexpression of the kiwifruit SVP3 gene affects reproductive development and suppresses anthocyanin biosynthesis in petals, but has no effect on vegetative growth, dormancy, or flowering time.
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DOI：
10.1093/jxb/eru264
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发表时间：
2014-09
影响因子：
6.9
通讯作者：
Varkonyi-Gasic E
中科院分区：
文献类型：
--
作者：
Wu R;Wang T;McGie T;Voogd C;Allan AC;Hellens RP;Varkonyi-Gasic E
SVP3基因过表达影响猕猴桃花和果实发育。减少花瓣色素沉着的结果干扰猕猴桃花组织特异性R2R3 MYB调节转录。SVP样MADS结构域转录因子在一年生植物中调节开花时间以及花序和花的发育，而在多年生植物中对生长停止和顶芽形成有影响。以前，在木本多年生藤本猕猴桃（猕猴桃属）中描述了四个SVP基因，在芽休眠和开花中可能有不同的作用。猕猴桃SVP3转录本被限制在营养组织中，并作为开花的阻遏物，因为它能够拯救拟南芥svp41突变体。为了进一步表征猕猴桃SVP 3，在猕猴桃物种中进行了异位表达。SVP3在A. deliciosa不影响一般植物生长或内休眠的持续时间。SVP3在A.毛蕊花还导致植物具有正常的营养生长、芽断裂和开花时间。然而，显着延长和异常的花，果实和种子发育观察，所产生的SVP3与猕猴桃花同源异型MADS结构域蛋白的相互作用。由于SVP3介导的干扰猕猴桃花组织特异性R2R3 MYB调节因子MYB110a和编码花青素生物合成关键步骤F3GT 1的基因的转录，花瓣色素沉着减少。SVP3的组成型表达对转基因烟草的生殖发育也有类似的影响。开花时间不受影响的光周期反应型品种，但开花和种子萌发显着延迟。花青苷在花瓣中的积累减少，并且证明了与R2R3 MYB NtAN 2转录物减少相同的潜在机制。
Overexpression of SVP3 affects kiwifruit flower and fruit development. The reduced petal pigmentation results from interference with transcription of the kiwifruit flower tissue-specific R2R3 MYB regulator. SVP-like MADS domain transcription factors have been shown to regulate flowering time and both inflorescence and flower development in annual plants, while having effects on growth cessation and terminal bud formation in perennial species. Previously, four SVP genes were described in woody perennial vine kiwifruit (Actinidia spp.), with possible distinct roles in bud dormancy and flowering. Kiwifruit SVP3 transcript was confined to vegetative tissues and acted as a repressor of flowering as it was able to rescue the Arabidopsis svp41 mutant. To characterize kiwifruit SVP3 further, ectopic expression in kiwifruit species was performed. Ectopic expression of SVP3 in A. deliciosa did not affect general plant growth or the duration of endodormancy. Ectopic expression of SVP3 in A. eriantha also resulted in plants with normal vegetative growth, bud break, and flowering time. However, significantly prolonged and abnormal flower, fruit, and seed development were observed, arising from SVP3 interactions with kiwifruit floral homeotic MADS-domain proteins. Petal pigmentation was reduced as a result of SVP3-mediated interference with transcription of the kiwifruit flower tissue-specific R2R3 MYB regulator, MYB110a, and the gene encoding the key anthocyanin biosynthetic step, F3GT1. Constitutive expression of SVP3 had a similar impact on reproductive development in transgenic tobacco. The flowering time was not affected in day-neutral and photoperiod-responsive Nicotiana tabacum cultivars, but anthesis and seed germination were significantly delayed. The accumulation of anthocyanin in petals was reduced and the same underlying mechanism of R2R3 MYB NtAN2 transcript reduction was demonstrated.
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影响因子：
4.2
作者：
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通讯作者：
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影响因子：
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作者：
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