Auxin-induced AUXIN RESPONSE FACTOR4 activates APETALA1 and FRUITFULL to promote flowering in woodland strawberry.
Auxin-induced AUXIN RESPONSE FACTOR4 activates APETALA1 and FRUITFULL to promote flowering in woodland strawberry.
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生长素诱导的 AUXIN RESPONSE FACTOR4 激活 APETALA1 和 FRUITFULL 促进林地草莓开花
DOI：
10.1038/s41438-021-00550-x
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发表时间：
2021-05-01
影响因子：
8.7
通讯作者：
Zhang Z
中科院分区：
文献类型：
--
作者：
Dong X;Li Y;Guan Y;Wang S;Luo H;Li X;Li H;Zhang Z
已知开花时间受许多途径调节，例如自主途径、赤霉素途径、老化途径、光周期介导途径和春化途径。这些调节机制涉及环境触发和内源性激素线索。由其他植物激素如生长素介导的其他开花控制机制还不太清楚。结果表明，生长素反应因子4（FaARF4）在栽培草莓（Fragaria× ananassa）中的表达量在开花期高于营养生长期。FaARF4在拟南芥和草莓中的过表达导致转基因植株开花早于对照植株。此外，与对照植物相比，FveARF4沉默的草莓植物显示延迟开花，表明FaARF4和FveARF4在调节开花方面的功能相似。进一步的研究表明，ARF4可以与花分生组织身份基因APETALA1（AP1）和FRUITFULL（FUL）的启动子结合，诱导其表达，从而在林地草莓中开花。我们的研究揭示了一个生长素介导的开花途径在草莓涉及诱导ARF 4的表达。
Flowering time is known to be regulated by numerous pathways, such as the autonomous, gibberellin, aging, photoperiod-mediated, and vernalization pathways. These regulatory mechanisms involve both environmental triggers and endogenous hormonal cues. Additional flowering control mechanisms mediated by other phytohormones, such as auxin, are less well understood. We found that in cultivated strawberry (Fragaria× ananassa), the expression of auxin response factor4 (FaARF4) was higher in the flowering stage than in the vegetative stage. Overexpression of FaARF4 in Arabidopsis thaliana and woodland strawberry (Fragaria vesca) resulted in transgenic plants flowering earlier than control plants. In addition, FveARF4-silenced strawberry plants showed delayed flowering compared to control plants, indicating that FaARF4 and FveARF4 function similarly in regulating flowering. Further studies showed that ARF4 can bind to the promoters of the floral meristem identity genes APETALA1 (AP1) and FRUITFULL (FUL), inducing their expression and, consequently, flowering in woodland strawberry. Our studies reveal an auxin-mediated flowering pathway in strawberry involving the induction of ARF4 expression.
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影响因子：
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