Dispersion of a Nanoliter Bolus in Microfluidic Co-Flow.
Dispersion of a Nanoliter Bolus in Microfluidic Co-Flow.
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DOI：
10.1088/0960-1317/24/3/034006
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发表时间：
2014-03
期刊：
影响因子：
--
通讯作者：
Fiering J
中科院分区：
文献类型：
--
作者：
Conway AJ;Saadi WM;Sinatra FL;Kowalski G;Larson D;Fiering J
微流体系统可以在纳升的尺度上进行反应和分析。然而，在这种尺度下，样品交付的不均匀性变得显著。为了确定特定设备所需的基本最小样本量，需要对质量传输有详细的了解。共流层流广泛应用于许多设备中，但通常仅在稳态下使用。由于建立共流稳态会消耗多余的样品体积和时间，因此在瞬态状态下操作设备是有利的，随着共流反应器体积的减小，瞬态状态下的操作设备占主导地位。对共流暂态的分析一直被忽视。在这项工作中，我们描述了一个气动控制的微流体喷射器的制造，该喷射器的构造是将一个离散的50nL丸注入双流共流反应器的一侧。使用染料进行图像分析，在0.5-10μL/min的流速范围内进行注射，为了比较，我们收集了该范围内的共流稳态数据。将图像分析结果与理论和仿真结果进行了比较，以进行设备验证。为了进行评估，我们建立了一个度量，表明在注射量中质量分布近似于稳态共流的程度。使用这种分析，瞬态注射可以在预定义的误差范围内近似于稳态条件，从而可以在减少试剂消耗的情况下直接进行测量。
Microfluidic systems enable reactions and assays on the scale of nanoliters. However, at this scale nonuniformities in sample delivery become significant. To determine the fundamental minimum sample volume required for a particular device, a detailed understanding of mass transport is required. Co-flowing laminar streams are widely used in many devices, but typically only in the steady-state. Because establishing the co-flow steady-state consumes excess sample volume and time, there is a benefit to operating devices in the transient state, which predominates as the volume of the co-flow reactor decreases. Analysis of the co-flow transient has been neglected thus far. In this work we describe the fabrication of a pneumatically controlled microfluidic injector constructed to inject a discrete 50nL bolus into one side of a two-stream co-flow reactor. Using dye for image analysis, injections were performed at a range of flow rates from 0.5-10μL/min, and for comparison we collected the co-flow steady-state data for this range. The results of the image analysis were also compared against theory and simulations for device validation. For evaluation, we established a metric that indicates how well the mass distribution in the bolus injection approximates steady-state co-flow. Using such analysis, transient-state injections can approximate steady-state conditions within predefined errors, allowing straight forward measurements to be performed with reduced reagent consumption.
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