Regional cellular heterogeneity and DNA synthetic activity in rat ventral prostate during postnatal development.
Regional cellular heterogeneity and DNA synthetic activity in rat ventral prostate during postnatal development.
复制标题
出生后发育过程中大鼠腹侧前列腺的区域细胞异质性和 DNA 合成活性。
DOI：
10.1095/biolreprod45.5.773
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发表时间：
1991
影响因子：
3.6
通讯作者：
Zirkin,BR
中科院分区：
文献类型：
--
作者：
Banerjee,PP;Banerjee,S;Sprando,RL;Zirkin,BR
以前的研究提供了证据表明，大鼠腹侧前列腺生长主要，如果不是唯一的，在其远端。然而，迄今为止，还没有分析中，在前列腺导管系统的定义区域的单个细胞已被解决和量化。此外，前列腺可能会在出生后的不同时间不同的增长的可能性已经得到很少的attention. We的目标是确定和量化的增殖上皮细胞和基质细胞在大鼠腹侧前列腺在其出生后的发展过程中的定义区域。为此，对10 - 60日龄的大鼠体内给予3 H-胸苷。然后使用解剖技术，通过该技术将前列腺导管系统的远端、中间和近端段彼此物理隔离，而不去除基质组织。对这些节段的纵切片进行细胞组成和DNA合成活性检查。观察到细胞组成和细胞增殖的区域异质性。在所有年龄段的大鼠中，在整个前列腺的上皮细胞和基质细胞中观察到DNA合成活性，而不仅仅是在远段。在第10天和第20天，远段标记的上皮细胞和基质细胞百分比显著高于近段;但在第45天和第60天，远段、中间段和近段标记的上皮细胞和基质细胞百分比相似。因此，在所有节段和所有年龄段中，在整个前列腺中观察到大量标记。这些数据表明，前列腺的长度和宽度在整个出生后的发展，让人联想到一棵树的生长。
Previous studies have provided evidence that the rat ventral prostate grows primarily, if not exclusively, at its distal tips. However, as yet there have been no analyses in which individual cells in defined regions of the prostatic ductal system have been resolved and quantified. Moreover, the possibility that the prostate might grow differently at different times of postnatal development has received little attention.Our objectives were to identify and quantify the proliferating epithelial and stromal cells in defined regions of the rat ventral prostate during its postnatal development. To this end,3H-thymidine was administered in vivo to rats of ages 10−60 days. A dissection technique was then used by which the distal, intermediate, and proximal segments of the prostatic ductal system were physically isolated from each other without removing the stromal tissue. Longitudinal sections of these segments were examined for cellular composition and DNA synthetic activity. Regional heterogeneity with respect to cell composition and cell proliferation was seen. In rats of all ages, DNA synthetic activity was seen in epithelial and stromal cells throughout the prostate, rather than only in the distal segment. At Days 10 and 20, significantly higher percentages of epithelial and stromal cells were labeled in the distal than in the proximal segments; but at Days 45 and 60, the percentages of labeled epithelial and stromal cells in the distal, intermediate, and proximal segments were similar. Thus, in all segments, and at all ages, substantial labeling was seen throughout the prostate. These data suggest that the prostate grows in both length and width throughout postnatal development, reminiscent of the growth of a tree.
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影响因子：
3.6
作者：
SUGIMURA, Y;CUNHA, GR;DONJACOUR, AA
通讯作者：
DONJACOUR, AA
影响因子：
3.6
作者：
SUGIMURA, Y;CUNHA, GR;BRODY, JR
通讯作者：
BRODY, JR
影响因子：
3.6
作者：
SUGIMURA, Y;CUNHA, GR;DONJACOUR, AA
通讯作者：
DONJACOUR, AA
发育中的大鼠前列腺中雄激素受体的表达不会因去势、氟他胺或肾上腺轴抑制而改变。
影响因子：
4.8
作者：
Husmann,DA;McPhaul,MJ;Wilson,JD
通讯作者：
Wilson,JD
大鼠腹侧前列腺细胞质雄激素受体的个体发育。
影响因子：
3.6
作者：
Jacob Rajfer;P. Namkung;Philip H. Petra
通讯作者：
Philip H. Petra