Cellular localization of Arabidopsis EARLY FLOWERING3 is responsive to light quality.
Cellular localization of Arabidopsis EARLY FLOWERING3 is responsive to light quality.
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DOI：
10.1093/plphys/kiac072
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发表时间：
2022-09-28
期刊：
影响因子：
7.4
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中科院分区：
文献类型：
--
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昼夜节律时钟有助于协调生理和发育过程以适应不断变化的日常和季节周期。拟南芥 (Arabidopsis thaliana) 生物钟中环境信号通路的枢纽是傍晚复合物 (EC)，这是一种由 EARLY FLOWERING3 (ELF3)、ELF4 和 LUX ARRYTHMO (LUX) 组成的蛋白质复合物。 EC 的形成取决于 ELF3，ELF3 是一种支架蛋白，可招募 EC 的其他成分和染色质重塑酶来抑制基因表达。因此，调节 ELF3 的细胞分布是控制其活性的重要机制。在这里，我们确定 ELF3 的细胞和亚核定位对红光 (RL) 和蓝光有响应，并且这两种波长对 ELF3 在细胞中的定位具有明显的竞争效应。我们进一步表征了 RL 反应，揭示至少有两条 RL 途径影响 ELF3 的细胞定位。其中之一取决于 RL 光感受器光敏色素 B (phyB)，而第二个至少部分独立于 phyB 活性。最后，我们研究了 ELF3 细胞定位的变化如何与 EC 靶基因表达的抑制相关。我们的分析揭示了一种复杂的效应，即需要 ELF3 来控制早相基因的 RL 敏感性，而不是晚相基因的 RL 敏感性。总之，我们的研究结果建立了一种以前未知的机制，光信号通过该机制影响 ELF3 活性。光信号通路通过控制夜间复合支架蛋白 EARLY FLOWERING3 的细胞和亚细胞定位并调节生物钟基因表达而汇聚在该蛋白上。
Circadian clocks facilitate the coordination of physiological and developmental processes to changing daily and seasonal cycles. A hub for environmental signaling pathways in the Arabidopsis (Arabidopsis thaliana) circadian clock is the evening complex (EC), a protein complex composed of EARLY FLOWERING3 (ELF3), ELF4, and LUX ARRYTHMO (LUX). Formation of the EC depends on ELF3, a scaffold protein that recruits the other components of the EC and chromatin remodeling enzymes to repress gene expression. Regulating the cellular distribution of ELF3 is thus an important mechanism in controlling its activity. Here, we determined that the cellular and sub-nuclear localization of ELF3 is responsive to red (RL) and blue light and that these two wavelengths have apparently competitive effects on where in the cell ELF3 localizes. We further characterized the RL response, revealing that at least two RL pathways influence the cellular localization of ELF3. One of these depends on the RL photoreceptor phytochrome B (phyB), while the second is at least partially independent of phyB activity. Finally, we investigated how changes in the cellular localization of ELF3 are associated with repression of EC target-gene expression. Our analyses revealed a complex effect whereby ELF3 is required for controlling RL sensitivity of morning-phased genes, but not evening-phased genes. Together, our findings establish a previously unknown mechanism through which light signaling influences ELF3 activity. Light signaling pathways converge on the evening complex scaffold protein EARLY FLOWERING3 by controlling its cellular and sub-cellular localization and regulating circadian clock gene expression.
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