A cross-sectional survey of bacterial species in plaque from client owned dogs with healthy gingiva, gingivitis or mild periodontitis.
A cross-sectional survey of bacterial species in plaque from client owned dogs with healthy gingiva, gingivitis or mild periodontitis.
复制标题
DOI：
10.1371/journal.pone.0083158
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Harris S
中科院分区：
文献类型：
--
作者：
Davis IJ;Wallis C;Deusch O;Colyer A;Milella L;Loman N;Harris S
牙周病是狗中最普遍的口腔疾病，如果不及时治疗，会导致宠物严重疼痛和牙齿脱落。本研究的目的是确定犬牙菌斑中与健康、牙龈炎和轻度牙周炎（<25%的附着丧失）显著相关的细菌种类。本研究收集了223只健康牙龈、牙龈炎和轻度牙周炎犬的龈下菌斑样本，每个健康状态有72 - 77个样本。从斑块样本中提取DNA，对16S rDNA的V1-V3区域进行PCR扩增。经生物信息学和统计学分析，对扩增子进行焦磷酸测序，共鉴定出274个操作分类单位。卟啉单胞菌是所有疾病阶段中数量最多的属，特别是在健康阶段，与莫拉氏菌和伯格氏菌并列。胃链球菌、放线菌和胃链球菌科是轻度牙周炎中最丰富的属。Logistic回归分析确定了这些属中与健康、牙龈炎或轻度牙周炎显著相关的物种。主成分分析显示出不同的社区健康和疾病概况。已确定的物种与人类的健康和牙周病有一些相似之处，但也有重大差异。与人类相比，健康犬菌斑被发现以革兰氏阴性细菌种类为主，而革兰氏阳性厌氧菌种类在疾病中占主导地位。这项研究的规模超过了以前发表的研究，增强了我们对犬龈下菌斑中细菌种类及其与健康和早期牙周病的关系的理解。
Periodontal disease is the most widespread oral disease in dogs which if left untreated results in significant pain to the pet and loss of dentition. The objective of this study was to identify bacterial species in canine plaque that are significantly associated with health, gingivitis and mild periodontitis (<25% attachment loss). In this survey subgingival plaque samples were collected from 223 dogs with healthy gingiva, gingivitis and mild periodontitis with 72 to 77 samples per health status. DNA was extracted from the plaque samples and subjected to PCR amplification of the V1-V3 region of the 16S rDNA. Pyrosequencing of the PCR amplicons identified a total of 274 operational taxonomic units after bioinformatic and statistical analysis. Porphyromonas was the most abundant genus in all disease stages, particularly in health along with Moraxella and Bergeyella. Peptostreptococcus, Actinomyces, and Peptostreptococcaceae were the most abundant genera in mild periodontitis. Logistic regression analysis identified species from each of these genera that were significantly associated with health, gingivitis or mild periodontitis. Principal component analysis showed distinct community profiles in health and disease. The species identified show some similarities with health and periodontal disease in humans but also major differences. In contrast to human, healthy canine plaque was found to be dominated by Gram negative bacterial species whereas Gram positive anaerobic species predominate in disease. The scale of this study surpasses previously published research and enhances our understanding of the bacterial species present in canine subgingival plaque and their associations with health and early periodontal disease.
登录
查看更多内容
影响因子：
14.9
作者：
Pruesse E;Quast C;Knittel K;Fuchs BM;Ludwig W;Peplies J;Glöckner FO
通讯作者：
Glöckner FO
影响因子：
48
作者：
Quince, Christopher;Lanzen, Anders;Sloan, William T.
通讯作者：
Sloan, William T.
犬口腔微生物组。
影响因子：
3.7
作者：
Dewhirst FE;Klein EA;Thompson EC;Blanton JM;Chen T;Milella L;Buckley CM;Davis IJ;Bennett ML;Marshall-Jones ZV
通讯作者：
Marshall-Jones ZV
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
3.3
作者：
Sturgeon, Amy;Stull, Jason W.;Weese, J. Scott
通讯作者：
Weese, J. Scott