Human cervicovaginal fluid biomarkers to predict term and preterm labor.
Human cervicovaginal fluid biomarkers to predict term and preterm labor.
复制标题
人宫颈阴道流体生物标志物可预测术语和早产。
DOI：
10.3389/fphys.2015.00151
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发表时间：
2015
影响因子：
4
通讯作者：
Georgiou HM
中科院分区：
文献类型：
--
作者：
Heng YJ;Liong S;Permezel M;Rice GE;Di Quinzio MK;Georgiou HM
早产（PTB；在妊娠37周之前出生）仍然是新生儿发病和死亡的主要原因。目前预测肺结核的生物标记物的效用有限。在怀孕期间，人宫颈阴道液（CVF）蛋白质组反映了局部生化环境，并受到阴道、子宫颈和相邻上覆胎膜发生的物理变化的影响。足月和早产（PTL）有共同的途径，宫颈成熟，子宫肌活化和胎膜破裂导致分娩。因此，我们假设预测分娩的CVF生物标志物在足月和早产环境中可能相似。在这篇综述中，我们总结了一些现有的已发表的文献，以及我们团队利用CVF发现和验证预测人类劳动的假定CVF生物标志物的广度。我们的团队建立了一种高效的方法来收集连续的CVF样品，以获得最佳的二维凝胶电泳分辨率和分析。我们首先开始了CVF生物标志物的发现，用于使用2d电泳和溶液阵列多分析物分析来预测足月分娩的自发发生。随后对与PTB相关的CVF样品进行2d电泳分析。一些蛋白质已被成功验证，并证明这些生物标志物与足月和PTL相关，并可能预测足月和PTL。此外，这些假定的生物标志物的测量被发现对阴道微生物群和/或精液的影响是稳健的。多种生物标志物床边测试的未来发展将有助于改善PTB的预测和患者的临床管理。
Preterm birth (PTB; birth before 37 completed weeks of gestation) remains the major cause of neonatal morbidity and mortality. The current generation of biomarkers predictive of PTB have limited utility. In pregnancy, the human cervicovaginal fluid (CVF) proteome is a reflection of the local biochemical milieu and is influenced by the physical changes occurring in the vagina, cervix and adjacent overlying fetal membranes. Term and preterm labor (PTL) share common pathways of cervical ripening, myometrial activation and fetal membranes rupture leading to birth. We therefore hypothesize that CVF biomarkers predictive of labor may be similar in both the term and preterm labor setting. In this review, we summarize some of the existing published literature as well as our team's breadth of work utilizing the CVF for the discovery and validation of putative CVF biomarkers predictive of human labor. Our team established an efficient method for collecting serial CVF samples for optimal 2-dimensional gel electrophoresis resolution and analysis. We first embarked on CVF biomarker discovery for the prediction of spontaneous onset of term labor using 2D-electrophoresis and solution array multiple analyte profiling. 2D-electrophoretic analyses were subsequently performed on CVF samples associated with PTB. Several proteins have been successfully validated and demonstrate that these biomarkers are associated with term and PTL and may be predictive of both term and PTL. In addition, the measurement of these putative biomarkers was found to be robust to the influences of vaginal microflora and/or semen. The future development of a multiple biomarker bed-side test would help improve the prediction of PTB and the clinical management of patients.
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DOI：
10.1016/s0002-9378(99)70609-5
发表时间：
1999-05-01
影响因子：
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作者：
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DOI：
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发表时间：
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