Morphological characterization of postembryonic development of blood-spleen barrier in duck.
Morphological characterization of postembryonic development of blood-spleen barrier in duck.
复制标题
鸭血脾屏障胚胎后发育的形态学特征
DOI：
10.1016/j.psj.2020.05.012
复制
发表时间：
2020-08
期刊：
影响因子：
4.4
通讯作者：
Chen Q
中科院分区：
文献类型：
--
作者：
Xu M;Li W;Yang S;Sun X;Tarique I;Yang P;Chen Q
脾是最大的外周淋巴器官和免疫反应的重要部位，血脾屏障(BSB)在抵抗各种病原体中起着重要作用。鸭脾的BSB结构与鸡和哺乳动物的不同。然而，在鸭子身上还没有关于胚胎后阶段BSB发育的信息。本研究对1日龄、7日龄、14日龄、21日龄、35日龄和60日龄雏鸭的脾进行了光镜和电子显微镜观察，以分析细胞结构的发育。结果表明，从1日龄到14日龄，鸭的脾指数呈不断增加的趋势。雏鸭早期脾内无明显的白髓和红髓带，但白髓面积大于红髓。35日龄鸭脾的椭圆形直径不断增大，1日龄后椭圆形淋巴鞘和动脉周围淋巴鞘继续发育，网状纤维随着年龄的增长而发展，其分支到达椭球壁，在14日龄的BSB中显示出一个发达的框架。7d后，被鞘毛细血管内皮细胞呈典型的立方体形状，细胞间间隙随日龄增加而增大，35日龄时基底膜和胶原纤维厚度增加。静脉注射BSB的机械过滤功能显示1日龄鸭脾白髓中有1层碳粒，而14~60d时，这些碳粒被限制在椭球和凝胶中，形成2层碳粒环。总体而言，鸭子BSB的细胞特征发育到胚胎后35d，以执行它们的免疫功能。
The spleen is the largest peripheral lymphoid organ and an important site of immune response, in which the blood–spleen barrier (BSB) plays a significant role to resist various pathogens. The BSB structure of duck spleen is different from that of chicken and mammals. However, no information about the development of BSB after the postembryonic age has been reported in ducks. The current study observed the spleen of 1, 7, 14, 21, 35, and 60-day-old ducks by light and electron microscopy to analyze the cellular structural development. The results showed that the spleen index was continuously increased from 1 to 14-day-old ducks. During their early age, the spleen of ducks showed no definite zone of white and red pulp, but the area of the white pulp was large compared to that of the red pulp. The diameter of the ellipsoid was constantly increased in up to 35-day-old duck spleen, while the periellipsoidal lymphatic sheath (PELS) and periarterial lymphatic sheath continuously developed after 1 D. The reticular fibers developed with age; their branching reached the ellipsoidal wall to show a developed framework in the BSB of 14-day-old ducks. After 7 D, the endothelial cells of the sheathed capillary showed a typical cuboidal shape; between these cells, the gaps increased as age advanced, while the thickness of the basement membrane and collagen fibers increased in 35-day-old ducks. The mechanical filtration function of BSB by intravenous injection showed a 1-layer ring of carbon particles restricted in the white pulp in 1-day-old duck spleen; however, in 14 to 60 D, these particles were restricted in the ellipsoid and PELS, forming 2-layer rings of carbon particles. Collectively, the cellular features of the duck BSB developed up to 35 D of postembryonic age to perform their immune function.
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影响因子：
3.6
作者：
BRELINSKA, R;PILGRIM, C;REISERT, I
通讯作者：
REISERT, I
DOI：
10.1038/nri3622
发表时间：
2014-03
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
通讯作者：
--
影响因子：
2.5
作者：
Ciriaco, E;Píñera, PP;Laurà, R
通讯作者：
Laurà, R
DOI：
10.1007/bf02890368
发表时间：
1983-01-01
期刊：
VIRCHOWS ARCHIV B-CELL PATHOLOGY INCLUDING MOLECULAR PATHOLOGY
影响因子：
--
作者：
FUJITA, H;KAWAMATA, S;YAMASHITA, K
通讯作者：
YAMASHITA, K
影响因子：
2.7
作者：
Wang, M. Z.;Ding, L. Y.;Zhang, J.
通讯作者：
Zhang, J.