Cytokine dysregulation in early- and late-term placentas from feline immunodeficiency virus (FIV)-infected cats.
Cytokine dysregulation in early- and late-term placentas from feline immunodeficiency virus (FIV)-infected cats.
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DOI：
10.1111/j.1600-0897.2010.00919.x
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发表时间：
2011-05
期刊：
影响因子：
--
通讯作者：
Coats KS
中科院分区：
文献类型：
--
作者：
Scott VL;Boudreaux CE;Lockett NN;Clay BT;Coats KS
实验性感染FIV-B-2542的猫产生高的胎儿感染率和生殖失败。我们假设fiv感染的皇后胎盘细胞因子表达失调，异常表达会加剧炎症并影响妊娠结局。我们的目的是量化早期和晚期胎盘组织中具有代表性的促炎细胞因子（IL-6、IL-12p35和IL-1β）、IL-10（抗炎）和趋化因子SDF-1α的表达。Real-time逆转录酶- pcr检测胎盘组织中基因表达。妊娠早期fiv感染组织中IL-6、IL-12p35表达升高，IL-10表达降低；妊娠后期，IL-6表达升高，IL-1β和SDF-1α表达降低。妊娠后期，IL-6表达与FIV载量呈正相关。IL-12:IL-10比值在妊娠早期高于妊娠晚期。在妊娠晚期，IL-12p35和SDF-1α表达降低，IL-12:IL-10比值降低。FIV感染在妊娠早期引起促炎性胎盘微环境，但在妊娠晚期没有。
Experimental infection of cats with FIV-B-2542 produces high rates of fetal infection and reproductive failure. We hypothesized that dysregulation of placental cytokine expression occurs in FIV-infected queens, and aberrant expression potentiates inflammation and impacts pregnancy outcome. Our purpose was to quantify expression of representative pro-inflammatory cytokines (IL-6, IL-12p35, and IL-1β), IL-10 (anti-inflammatory), and the chemokine SDF-1α in early- and late-term placental tissues. Real-time reverse-transcriptase-PCR was used to measure gene expression in placental tissues. Increased expression of IL-6 and IL-12p35 and decreased expression of IL-10 occurred in FIV-infected tissues at early pregnancy; at late gestation, IL-6 expression increased and IL-1β and SDF-1α decreased. At late pregnancy, IL-6 expression positively correlated with FIV load. IL-12:IL-10 ratios were higher in infected tissues at early, but not late pregnancy. Fetal nonviability accompanied decreased IL-12p35 and SDF-1α expression at both stages and decreased IL-12:IL-10 ratio at late pregnancy. FIV infection caused a pro-inflammatory placental microenvironment at early, but not late pregnancy.
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