Effects of Oral Glutamine Supplementation, Birthweight and Age on Colonic Morphology and Microbiome Development in Male Suckling Piglets.
Effects of Oral Glutamine Supplementation, Birthweight and Age on Colonic Morphology and Microbiome Development in Male Suckling Piglets.
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DOI：
10.3390/microorganisms10101899
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发表时间：
2022-09-25
期刊：
影响因子：
4.5
通讯作者：
Zentek J
中科院分区：
文献类型：
--
作者：
Schulze Holthausen J;Schregel J;Sciascia QL;Li Z;Tuchscherer A;Vahjen W;Metges CC;Zentek J
哺乳低出生体重仔猪的死亡率、发育受损和代谢功能障碍可能受到通过补充谷氨酰胺调节肠道微生物组的影响。因此，本研究检查了谷氨酰胺补充是否会影响5日龄和12日龄雄性低出生体重和正常出生体重仔猪的结肠发育和微生物组组成。哺乳仔猪经口补充谷氨酰胺或丙氨酸。获取结肠消化物样本进行16 S rDNA测序、细菌代谢产物测定和组织形态学组织分析。与补充丙氨酸的仔猪相比，补充谷氨酰胺的仔猪结肠直肠中的尸胺和亚精胺浓度较低（p < 0.05），而CD3+结肠上皮内淋巴细胞数量较高（p < 0.05）。低出生体重仔猪的特征在于厚壁菌门、阴性芽孢杆菌属和粪杆菌属的相对丰度较低，而Alistipes的丰度较高（p < 0.05）。与正常出生体重仔猪相比，低出生体重仔猪的尸胺和总生物胺浓度（p < 0.05）以及CD3+上皮内淋巴细胞浓度（p < 0.05）较低。与年龄因素相比，谷氨酰胺补充剂和出生体重与结肠微生物和组织学特征的微小变化相关，这表明个体发育因素在肠道发育中发挥着更重要的作用。
Mortality, impaired development and metabolic dysfunctions of suckling low-birthweight piglets may be influenced by modulating the intestinal microbiome through glutamine supplementation. Therefore, this study examined whether glutamine supplementation may affect the colonic development and microbiome composition of male low- and normal-birthweight piglets at 5 and 12 days of age. Suckling piglets were supplemented orally with glutamine or alanine. Colonic digesta samples were obtained for 16S rDNA sequencing, determination of bacterial metabolites and histomorphological tissue analyses. Glutamine-supplemented piglets had lower concentrations of cadaverine and spermidine in the colonic digesta (p < 0.05) and a higher number of CD3+ colonic intraepithelial lymphocytes compared to alanine-supplemented piglets (p < 0.05). Low-birthweight piglets were characterised by a lower relative abundance of Firmicutes, the genera Negativibacillus and Faecalibacterium and a higher abundance of Alistipes (p < 0.05). Concentrations of cadaverine and total biogenic amines (p < 0.05) and CD3+ intraepithelial lymphocytes (p < 0.05) were lower in low- compared with normal-birthweight piglets. In comparison to the factor age, glutamine supplementation and birthweight were associated with minor changes in microbial and histological characteristics of the colon, indicating that ontogenetic factors play a more important role in intestinal development.
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影响因子：
7.3
作者：
Grondin JA;Kwon YH;Far PM;Haq S;Khan WI
通讯作者：
Khan WI
断奶过渡期间商品仔猪肠道微生物群的成熟发展
影响因子：
5.2
作者：
Chen L;Xu Y;Chen X;Fang C;Zhao L;Chen F
通讯作者：
Chen F
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
L-谷氨酰胺或 L-丙氨酰-L-谷氨酰胺可防止氧化剂或内毒素诱导的新生儿肠上皮细胞死亡
影响因子：
3.5
作者：
Haynes, Tony E.;Li, Peng;Wu, Guoyao
通讯作者：
Wu, Guoyao
影响因子：
2.7
作者：
Ferrara, F.;Tedin, L.;Zentek, J.
通讯作者：
Zentek, J.