The Immune Response in the Uteri and Placentae of Chlamydia abortus-Infected Ewes and Its Association with Pregnancy Outcomes.
The Immune Response in the Uteri and Placentae of Chlamydia abortus-Infected Ewes and Its Association with Pregnancy Outcomes.
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DOI：
10.3390/pathogens12060846
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发表时间：
2023-06-19
期刊：
影响因子：
--
通讯作者：
Longbottom D
中科院分区：
文献类型：
--
作者：
Caspe SG;Ewing DA;Livingstone M;Underwood C;Milne E;Sargison ND;Wattegedera SR;Longbottom D
由流产衣原体（Chlamydia abortus，C.流产），是绵羊流产的主要原因之一。有多种因素，包括衣原体生长，宿主免疫反应和激素平衡，导致不同的妊娠结果，如流产，可能死亡的弱羔羊或健康羔羊的出生。本研究旨在确定双胞胎绵羊（两只羔羊出生时都死了;一只活的，一只死的;两只都活的）实验性感染C时免疫细胞浸润表型模式与不同妊娠结果之间的关系。流产分娩后收集绵羊子宫和胎盘。通过免疫组织化学和原位杂交分析所有样品的特异性免疫细胞特征，包括细胞表面抗原和T调节（Treg）细胞相关转录因子和细胞因子。其中一些免疫抗原首次在绵羊生殖组织中进行了评价。辅助性T细胞/调节性T细胞的差异模式揭示了胎盘中的显著组效应。提示淋巴细胞亚群的平衡可能在影响C.流产感染的羊本研究提供了新的详细信息，在母胎界面上观察到的免疫反应在羊早产流产或产羔的时间。
The enzootic abortion of ewes, caused by the bacterium Chlamydia abortus (C. abortus), is one of the main causes of abortion in sheep. There are multiple contributory factors, including chlamydial growth, host immune response, and hormonal balance, that result in different pregnancy outcomes, such as abortion, the birth of weak lambs that may die, or healthy lambs. This study aimed to determine the relationship between phenotypical patterns of immune cell infiltration and different pregnancy outcomes in twin-bearing sheep (both lambs born dead; one alive and one dead; both alive) when experimentally infected with C. abortus. Both the sheep uteri and placentae were collected after parturition. All samples were analysed for specific immune cell features, including cell surface antigens and the T-regulatory (Treg) cell-associated transcription factor and cytokines, by immunohistochemistry and in situ hybridisation. Some of these immunological antigens were evaluated in ovine reproductive tissues for the first time. Differential patterns of T helper/Treg cells revealed significant group effects in the placentae. It suggests the potential role that the balance of lymphocyte subsets may play in affecting different pregnancy outcomes in C. abortus-infected sheep. The present study provides novel detailed information about the immune responses observed at the maternofoetal interface in sheep at the time of pre-term abortion or lambing.
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影响因子：
4.6
作者：
Bankhead P;Loughrey MB;Fernández JA;Dombrowski Y;McArt DG;Dunne PD;McQuaid S;Gray RT;Murray LJ;Coleman HG;James JA;Salto-Tellez M;Hamilton PW
通讯作者：
Hamilton PW
影响因子：
5.2
作者：
Essig, Andreas;Longbottom, David
通讯作者：
Longbottom, David
反刍动物怀孕早期怀孕期间的中性粒细胞可能影响。
影响因子：
1.7
作者：
Fiorenza MF;Amaral CDS;da Anunciação ARA;Portela VVM;Marey MA;Miyamoto A;Antoniazzi AQ
通讯作者：
Antoniazzi AQ
影响因子：
3.4
作者：
Fox, Annette;Maddox, Jill F.;Meeusen, Els N.
通讯作者：
Meeusen, Els N.
影响因子：
2.2
作者：
Bartoskova, A.;Turanek-Knotigova, P.;Faldyna, M.
通讯作者：
Faldyna, M.