Enabling quantitative analysis in ambient ionization mass spectrometry: internal standard coated capillary samplers.
Enabling quantitative analysis in ambient ionization mass spectrometry: internal standard coated capillary samplers.
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DOI：
10.1021/ac401056q
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发表时间：
2013-06-18
影响因子：
7.4
通讯作者：
Ouyang, Zheng
中科院分区：
文献类型：
--
作者：
Liu, Jiangjiang;Cooks, R. Graham;Ouyang, Zheng
本文描述了一种采用环境电离质谱法对小体积复杂混合物（~1 μL）中痕量分析物进行定量分析的玻璃毛细管取样方法。取样玻璃毛细管的内表面涂有内部标准，然后用于抽取液体样品，从而将单个固定体积的分析物和内部标准转移到基板上进行分析。在此过程中，内标液自动混入样品中，内标液和样品的体积均由毛细管体积固定。定量精度对毛细管长度的变化不敏感，使采样毛细管的制备简单，并提供了一个可靠的采样方案。采用不同的环境电离方法，对1 μL样品的定量精度有显著提高。
We describe a sampling method using glass capillaries for quantitative analysis of trace analytes in small volumes of complex mixtures (~1 μL) using ambient ionization mass spectrometry. The internal surface of a sampling glass capillary was coated with internal standard then used to draw liquid sample and so transfer both the analyte and internal standard in a single fixed volume onto a substrate for analysis. The internal standard was automatically mixed into the sample during this process and the volumes of the internal standard solution and sample are both fixed by the capillary volume. Precision in quantitation is insensitive to variations in length of the capillary, making the preparation of the sampling capillary simple and providing a robust sampling protocol. Significant improvements in quantitation accuracy were obtained for analysis of 1 μL samples using various ambient ionization methods.
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DOI：
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发表时间：
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影响因子：
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作者：
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通讯作者：
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