Local Administration of Magnesium Promotes Meniscal Healing Through Homing of Endogenous Stem Cells: A Proof-of-Concept Study
Local Administration of Magnesium Promotes Meniscal Healing Through Homing of Endogenous Stem Cells: A Proof-of-Concept Study
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镁的局部给药通过内源干细胞归巢促进半月板愈合：概念验证研究
DOI：
10.1177/0363546518820076
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发表时间：
2019-02
期刊：
影响因子：
--
通讯作者：
Song Bin
中科院分区：
文献类型：
--
作者：
Zhang Zheng Zheng;Zhou Yun Feng;Li Wei Ping;Jiang Chuan;Chen Zhong;Luo Huan;Song Bin
背景：虽然已经开发了许多策略来改变半月板缝合修复的生物和生物力学环境，以提高愈合的机会，但失败率仍然很高。因此，新方法促进半月板。假设：镁的管理（通过使用镁缝线的修复）可能会增强愈合和粘附性。内源性干细胞移植到病变部位，从而促进半月板原位再生和软骨保护功能。研究设计：对照实验室研究。方法：从兔的膝关节中鉴定并分离滑膜液来源的间充质干细胞（SMSCs）。半月板损伤持续4周。对SMSCs的粘附性和趋化性进行了体外分析。对于in。体内实验将半月板病变兔（n = 120）分为3组：采用高纯镁缝线(Mg；组)、可吸收缝线修补（对照组）、不修补（空白组）。术后1周、3周、6周和12周通过大体和组织学分析评估再生组织的愈合情况和关节软骨的退变情况。的。对修复后半月板的力学性能进行了分析（拉伸试验）。结果：在体外，镁能促进SMSCs的粘附和迁移，并在膝关节中被发现和增加。有半月板病变的关节。此外，镁刺激SMSCs的纤维软骨形成。与另一个相比。Mg组小鼠组织再生、下软骨退变明显增强，机械强度保持在。半月板修复后12周…结论/临床意义：由于镁的潜在容量，镁可以用于半月板原位修复。招募内源性干细胞，促进纤维软骨基质的合成。
Background: Although many strategies have been developed to modify the biological and biomechanical environment of the meniscal suture repair to improve the chances of healing, the failure rates remain high. Thus, new methods to promote meniscal.regeneration and repair are needed..Hypothesis: Administration of magnesium (via a repair using magnesium stitches) might enhance recruitment and adherence of.endogenous stem cells to the site of the lesion, thereby promoting in situ meniscal regeneration and chondroprotective functions..Study Design: Controlled laboratory study..Methods: Synovial fluid–derived mesenchymal stem cells (SMSCs) were identified and isolated from the knees of rabbits with.a meniscal injury of 4 weeks’ duration. An in vitro analysis of adherence and chemotaxis of SMSCs was performed. For the in.vivo assay, rabbits (n = 120) with meniscal lesions were divided into 3 groups: repair with high-purity magnesium stitches (Mg.group), repair with absorbable sutures (Control group), and no repair (Blank group). Healing of the regenerated tissue and degeneration of the articular cartilage were evaluated by gross and histological analysis at postoperative weeks 1, 3, 6, and 12. The.mechanical properties of the repaired meniscus were also analyzed (tensile testing)..Results: In vitro, magnesium promoted the adhesion and migration of SMSCs, which were identified and increased in the knee.joints with meniscal lesions. Moreover, fibrochondrogenesis of SMSCs was stimulated by magnesium. Compared with the other.groups, the Mg group had enhanced tissue regeneration, lower cartilage degeneration, and retained mechanical strength at.12 weeks after meniscal repair..Conclusion/Clinical Relevance: Magnesium could be used for in situ meniscal repair due to the potential capacity of magnesium.to recruit endogenous stem cells and promote synthesis of fibrocartilaginous matrix.
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影响因子：
4.2
作者：
Matsukura Y;Muneta T;Tsuji K;Koga H;Sekiya I
通讯作者：
Sekiya I
DOI：
10.1016/j.arthro.2011.06.041
发表时间：
2011-12-01
影响因子：
4.7
作者：
Angel Ruiz-Iban, Miguel;Diaz-Heredia, Jorge;Abraira, Victor
通讯作者：
Abraira, Victor
影响因子：
--
作者：
Feyerabend, Frank;Witte, Frank;Willumeit, Regine
通讯作者：
Willumeit, Regine
影响因子：
4.8
作者：
Cinque, Mark E.;Geeslin, Andrew G.;LaPrade, Robert F.
通讯作者：
LaPrade, Robert F.
DOI：
10.1302/0301-620x.92b1.22629
发表时间：
2010-01-01
影响因子：
--
作者：
Dutton, A. Q.;Choong, P. F.;Hui, J. H. P.
通讯作者：
Hui, J. H. P.