Upregulated expression of N-methyl-D-aspartate receptor 1 and nitric oxide synthase during form-deprivation myopia in guinea pigs.
Upregulated expression of N-methyl-D-aspartate receptor 1 and nitric oxide synthase during form-deprivation myopia in guinea pigs.
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豚鼠形觉剥夺性近视期间 N-甲基-D-天冬氨酸受体 1 和一氧化氮合酶表达上调。
DOI：
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发表时间：
2015-04
期刊：
影响因子：
--
通讯作者：
刘菲
中科院分区：
文献类型：
--
作者：
文丹;宋伟涛;刘双珍;谭星平;刘菲
本研究旨在探讨N-甲基-D-天冬氨酸受体1（NMDAR 1）和神经元型一氧化氮合酶（ncNOS）在形觉剥夺性近视眼（FDM）中的表达。用豚鼠面罩建立FDM模型。免疫组化和Western blot检测视网膜NMDAR 1的表达。原位杂交检测ncNOS mRNA表达。放射免疫法测定cGMP含量。对照组双眼视网膜NMDAR 1和ncNOS表达，两眼间NMDAR 1和ncNOS表达及cGMP含量无显著性差异。而FDM组视网膜NMDAR 1、ncNOS表达及cGMP含量均显著高于对侧未治疗眼。ncNOSmRNA水平与cGMP含量呈高度相关。总之，FDM上调视网膜中NMDAR 1和ncNOS的表达，并增加cGMP含量。NMDAR 1/NO-cGMP通路可能参与近视进展过程中异常的视觉信号。
This study aimed to investigate the expression of N-methyl-D-aspartate receptor 1 (NMDAR1) and neuronal constitutive nitric oxide synthase (ncNOS) during form-deprivation myopia (FDM). FDM models were established in guinea pigs with facemasks. NMDAR1 expression in the retina was detected by immunohistochemistry and Western blot analysis. ncNOS mRNA expression was examined by in situ hybridization. cGMP content was measured by radioimmunoassay. In control group, NMDAR1 and ncNOS were expressed in binocular retinas, and there was no significant difference in NMDAR1 and ncNOS expression and cGMP content between the two eyes. However, NMDAR1 and ncNOS expression and cGMP content in the retina of FDM eyes were significantly higher than that of contralateral untreated eyes. Furthermore, ncNOS mRNA level and cGMP content was highly correlated. In conclusion, FDM upregulates the expression of NMDAR1 and ncNOS and increases cGMP content in the retina. NMDAR1/NO-cGMP pathway may contribute to abnormal visual signals during myopic progression.
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DOI：
10.1097/opx.0000000000000041
发表时间：
2013-11
期刊：
Optometry and vision science : official publication of the American Academy of Optometry
影响因子：
--
作者：
Nickla DL;Lee L;Totonelly K
通讯作者：
Totonelly K
影响因子：
2.9
作者：
Jie Wu;Qiong Liu;X. Yang;Hui Yang;Jun-wen Zeng
通讯作者：
Jie Wu;Qiong Liu;X. Yang;Hui Yang;Jun-wen Zeng
影响因子：
2
作者：
Fujikado, T;Kawasaki, Y;Tano, Y
通讯作者：
Tano, Y
影响因子：
1.7
作者：
Z. Yin;S. Crewther;Mo Yang;D. Crewther
通讯作者：
Z. Yin;S. Crewther;Mo Yang;D. Crewther
影响因子：
2.1
作者：
M. Sasoh;Ning Ma;Hisashi Matsubara;K. Matsunaga;Y. Uji
通讯作者：
M. Sasoh;Ning Ma;Hisashi Matsubara;K. Matsunaga;Y. Uji