Low-cost photolithographic fabrication of nanowires and microfilters for advanced bioassay devices.
Low-cost photolithographic fabrication of nanowires and microfilters for advanced bioassay devices.
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DOI：
10.3390/s150306091
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发表时间：
2015-03-12
期刊：
影响因子：
--
通讯作者：
Papadimitrakopoulos F
中科院分区：
文献类型：
--
作者：
Doan NM;Qiang L;Li Z;Vaddiraju S;Bishop GW;Rusling JF;Papadimitrakopoulos F
具有纳米尺寸阵列电极和微过滤能力的集成微流体装置可以大大提高灵敏度并增强免疫测定装置的自动化。在这方面的贡献，我们利用薄铝（Al）膜的边缘图案化方法，以形成纳米至微米尺寸的间隙。高功函金属的蒸发（即，Au、Ag等）在这些间隙上，随后进行Al剥离，使得能够从低成本的塑料基光掩模形成电均匀的纳米线。通过用铬（Cr）代替Al，实现了对于多个阵列器件的低成本制造是理想的高分辨率定制光掩模的形成。为了证明这种Cr光掩模的可行性，形成SU-8微柱母版并将其复制到PDMS中以产生具有3-4 μm间隙和3的纵横比的微米级滤光器。这些微滤器能够将6 µm微珠保留在局部位置，同时允许溶剂流动。纳米线阵列和微柱过滤的组合为各种基于微流体的免疫测定几何结构的快速研发筛选开辟了新的前景，其中分析物预浓缩和高灵敏度的电化学检测可以容易地共定位。
Integrated microfluidic devices with nanosized array electrodes and microfiltration capabilities can greatly increase sensitivity and enhance automation in immunoassay devices. In this contribution, we utilize the edge-patterning method of thin aluminum (Al) films in order to form nano- to micron-sized gaps. Evaporation of high work-function metals (i.e., Au, Ag, etc.) on these gaps, followed by Al lift-off, enables the formation of electrical uniform nanowires from low-cost, plastic-based, photomasks. By replacing Al with chromium (Cr), the formation of high resolution, custom-made photomasks that are ideal for low-cost fabrication of a plurality of array devices were realized. To demonstrate the feasibility of such Cr photomasks, SU-8 micro-pillar masters were formed and replicated into PDMS to produce micron-sized filters with 3–4 µm gaps and an aspect ratio of 3. These microfilters were capable of retaining 6 µm beads within a localized site, while allowing solvent flow. The combination of nanowire arrays and micro-pillar filtration opens new perspectives for rapid R&D screening of various microfluidic-based immunoassay geometries, where analyte pre-concentration and highly sensitive, electrochemical detection can be readily co-localized.
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影响因子：
17.1
作者：
Mani, Vigneshwaran;Chikkaveeraiah, Bhaskara V.;Patel, Vyomesh;Gutkind, J. Silvio;Rusling, James F.
通讯作者：
Rusling, James F.
影响因子：
8.4
作者：
Andersson, H;van der Wijngaart, W;Stemme, G
通讯作者：
Stemme, G
影响因子：
6.1
作者：
Ikami, Mai;Kawakami, Ayako;Baba, Yoshinobu
通讯作者：
Baba, Yoshinobu
影响因子：
17.1
作者：
Li, Jason;Zhang, Yanliang;Sun, Yu
通讯作者：
Sun, Yu
用于生物标志物多重测量和即时检测的三维纸基电化学发光免疫装置
影响因子：
14
作者：
Ge, Lei;Yan, Jixian;Yu, Jinghua
通讯作者：
Yu, Jinghua