Mitigating the Effects of Electrode Biofouling-Induced Impedance for Improved Long-Term Electrochemical Measurements In Vivo.
Mitigating the Effects of Electrode Biofouling-Induced Impedance for Improved Long-Term Electrochemical Measurements In Vivo.
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DOI：
10.1021/acs.analchem.9b05194
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发表时间：
2020-05-05
影响因子：
7.4
通讯作者：
Heien ML
中科院分区：
文献类型：
--
作者：
Seaton BT;Hill DF;Cowen SL;Heien ML
生物结垢是涉及在大脑中长时间植入电化学探针的研究中的普遍问题。在长期的快速扫描循环伏安法（FSCV）研究中，生物污垢表现为背景信号的峰值氧化电位的变化，该变化持续数天至数周，降低了对多巴胺等神经递质的敏感性和选择性。使用开路电位（OCP）测量，扫描电子显微镜/能量色散X射线光谱（SEM/EDX）和电化学阻抗谱（EIS），我们研究了由于电极植入而发生的生物污损诱导事件。我们确定，FSCV背景信号偏移是由Ag/AgCl线参比电极的阴极极化以及Ag/AgCl线参比电极和碳纤维工作电极的电化学阻抗增加引起的。这些事件可能是由免疫反应诱导的电极包囊共同引起的。阿headstage利用三电极配置，旨在补偿生物污垢的阻抗分量，减少了FSCV背景信号在体内的偏移，并保留多巴胺的敏感性，在人工增加的阻抗水平在体外。结合稳定的参考电极，这种三电极配置将是至关重要的，在长期FSCV研究期间实现可靠的神经递质检测。
Biofouling is a prevalent issue in studies that involve prolonged implantation of electrochemical probes in the brain. In long-term fast-scan cyclic voltammetry (FSCV) studies, biofouling manifests as a shift in the peak oxidative potential of the background signal that worsens over days to weeks, diminishing sensitivity and selectivity to neurotransmitters such as dopamine. Using open circuit potential (OCP) measurements, scanning electron microscopy/energy-dispersive X-ray spectroscopy (SEM/EDX), and electrochemical impedance spectroscopy (EIS), we examined the biofouling-induced events that occur due to electrode implantation. We determined that the FSCV background signal shift results from cathodic polarization of the Ag/AgCl-wire reference electrode and increased electrochemical impedance of both the Ag/AgCl-wire reference electrode and carbon-fiber working electrode. These events are likely caused collectively by immune response-induced electrode encapsulation. A headstage utilizing a three-electrode configuration, designed to compensate for the impedance component of biofouling, reduced the FSCV background signal shift in vivo and preserved dopamine sensitivity at artificially increased impedance levels in vitro. In conjunction with a stable reference electrode, this three-electrode configuration will be critical in achieving reliable neurotransmitter detection for the duration of long-term FSCV studies.
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影响因子：
5
作者：
Rodeberg NT;Sandberg SG;Johnson JA;Phillips PE;Wightman RM
通讯作者：
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影响因子：
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作者：
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DOI：
10.1007/978-1-62703-251-3_16
发表时间：
2013-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Roberts, James G;Lugo-Morales, Leyda Z;Sombers, Leslie A
通讯作者：
Sombers, Leslie A
超薄细胞膜模拟磷酸胆碱聚合物薄膜涂层可大幅改进体内电化学检测
影响因子：
16.6
作者：
Liu, Xiaomeng;Xiao, Tongfang;Mao, Lanqun
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