The embryonic form of neural cell surface molecule (E-NCAM) in the rat hippocampus and its reexpression on glial cells following kainic acid- induced status epilepticus
The embryonic form of neural cell surface molecule (E-NCAM) in the rat hippocampus and its reexpression on glial cells following kainic acid- induced status epilepticus
复制标题
大鼠海马神经细胞表面分子（E-NCAM）的胚胎形式及其在红藻氨酸诱导癫痫持续状态后在胶质细胞上的再表达
作者：
G. le gal la Salle;G. Rougon;A. Valin
神经细胞粘附分子（NCAM）在发育过程中在细胞表面发生变化，从高度唾液酸化的形式（胚胎或E-NCAM）到三种大小类别的唾液酸化程度较低的蛋白质，表观分子量为180，140和120 kDa（成人NCAM）。在神经系统中，E-NCAM已经定位于发育中的组织中，在那里它被认为在神经元组的结构化和组织模式形成中起作用。在本研究中，特异性检测E-NCAM的单克隆抗体用于免疫印迹和免疫组织化学程序。在发育中的大鼠海马中，发现E-NCAM细胞表达根据精确的模式变化，并持续到出生后1个月。它与苔藓纤维系统密切相关，这是一个以发芽倾向而闻名的区域。在成年大鼠中，虽然免疫反应性大大降低，变得无法检测到的免疫印迹分析，E-NCAM仍然被发现与一些齿状回的最里面的一部分的海绵状细胞。为了获得对E-NCAM的潜在组织遗传学可塑性功能的一些了解，在另一系列实验中，用红藻氨酸（一种引发癫痫持续状态的强大兴奋毒性和惊厥性谷氨酸类似物）处理成年大鼠。当检查这些动物的E-NCAM表达时，发现与神经胶质样细胞相关的强烈标记，特别是在海马结构中，并且大致对应于反应性神经胶质增生，如通过用抗神经胶质细胞酸性蛋白抗体染色所证实的。这种表达从红藻氨酸给药后约3天可检测到，并持续至少12周;它根据可观察到的时空分布模式发展。在杏仁核点燃，继发性全身性癫痫的非病变模型提交的动物，没有观察到这样的E-NCAM的再表达。我们的观察结果表明，聚唾液酸化可能是一种手段，确定神经元结构的可塑性，在中枢神经系统。此外，E-NCAM的强烈再表达可能是脑损伤后反应性胶质增生的标志物。
The neural cell adhesion molecule (NCAM) changes at the cell surface during development, from highly sialylated forms (embryonic or E-NCAM) to three size classes of less sialylated proteins with apparent molecular mass of 180, 140, and 120 kDa (adult NCAM). In the nervous system, E-NCAM has been localized in developing tissues, where it is thought to play a role in the structuring of neuronal groups and tissue pattern formation. In the present study a monoclonal antibody that specifically detects E-NCAM was used in immunoblot and immunohistochemical procedures. In developing rat hippocampus, E-NCAM cell expression was found to change according to a precise pattern and persisted until 1 month after birth. It was closely associated with the mossy fiber system, an area known for its sprouting propensity. In adult rats, although immunoreactivity considerably decreases and becomes undetectable by immunoblot analysis, E-NCAM was still found to be associated with a few pyramidal-shaped cells in the innermost part of the dentate gyrus. In order to acquire some insight into potential histogenetically plastic functions of E-NCAM, in another series of experiments adult rats were treated with kainic acid, a powerful excitotoxic and convulsant glutamate analog eliciting status epilepticus. When these animals were examined for E-NCAM expression, an intense labeling was found associated with glial-like cells, particularly in the hippocampal formation, and corresponding approximately to the reactive gliosis, as confirmed by staining with anti-glial fibrillary acidic protein antibodies. This expression was detectable from about 3 d following kainic acid administration and persisted for at least 12 weeks; it developed according to an observable spatiotemporal distribution pattern. In animals submitted to amygdala kindling, a nonlesional model of secondarily generalized epilepsy, no such reexpression of E-NCAM was observed. Our observations imply that polysialylation may be a means of identifying neuronal structures capable of plasticity in the CNS. Moreover, intense reexpression of E-NCAM could be a marker of reactive gliosis following brain damage.
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影响因子：
56.9
作者：
SUTULA, T;HE, XX;SCOTT, G
通讯作者：
SCOTT, G
影响因子：
56.9
作者：
SLOVITER, RS
通讯作者：
SLOVITER, RS
小鼠小脑和齿状回发育过程中 NCAM 基因的表达。
DOI：
10.1016/0165-3806(90)90230-v
发表时间：
1990
期刊：
Brain research. Developmental brain research
影响因子：
--
作者：
Goldowitz,D;Barthels,D;Lorenzon,N;Jungblut,A;Wille,W
通讯作者：
Wille,W
影响因子：
2.9
作者：
EDELMAN, GM
通讯作者：
EDELMAN, GM
影响因子：
56.9
作者：
RUTISHAUSER, U;ACHESON, A;SUNSHINE, J
通讯作者：
SUNSHINE, J