Identification of the genes that contribute to lactate utilization in Helicobacter pylori.
Identification of the genes that contribute to lactate utilization in Helicobacter pylori.
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DOI：
10.1371/journal.pone.0103506
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Yamaoka Y
中科院分区：
文献类型：
--
作者：
Iwatani S;Nagashima H;Reddy R;Shiota S;Graham DY;Yamaoka Y
幽门螺杆菌是一种革兰氏阴性、螺旋状的嗜氧微细菌，与胃癌的病因学相关。尽管在幽门螺杆菌基因组中还没有发现相应的基因，但乳酸的利用与幽门螺杆菌有关。在此，我们报道了hp0137-0139(LldEFG)、hp0140-0141(LctP)和hp1222(DLD)基因产物在幽门螺杆菌中对D-乳酸和L-乳酸的利用。幽门螺杆菌26695的三个基因单元hp0137-0139编码L乳酸脱氢酶，该酶催化乳酸转化为丙酮酸，并依赖于NAD。这些基因的同基因突变株不能在L乳酸依赖的培养基上生长。Hp1222基因产物是一种不依赖NAD的D-乳酸脱氢酶，也参与了L-乳酸的氧化；该基因的等基因突变体不能在D-乳酸依赖的培养基上生长。平行基因hp0140-0141编码两个几乎相同的乳酸膜(LCTP)，促进D-乳酸和L-乳酸的摄取。有趣的是，乳酸的运输也必须存在替代途径，因为基因敲除并不能完全阻止D-乳酸或L-乳酸的生长。乳酸作为碳源不能提高hp0137-0139和hp1222的基因表达水平。乳酸L对hp0140-0141的表达有轻微的抑制作用，但D-乳酸对hp0140-0141的表达没有影响。本研究确定了影响乳酸利用的基因，并证明了幽门螺杆菌同时利用D-乳酸和L-乳酸的能力。
Helicobacter pylori are Gram-negative, spiral-shaped microaerophilic bacteria etiologically related to gastric cancer. Lactate utilization has been implicated although no corresponding genes have been identified in the H. pylori genome. Here, we report that gene products of hp0137–0139 (lldEFG), hp0140–0141 (lctP), and hp1222 (dld) contribute to D- and L-lactate utilization in H. pylori. The three-gene unit hp0137–0139 in H. pylori 26695 encodes L-lactate dehydrogenase (LDH) that catalyzes the conversion of lactate to pyruvate in an NAD-dependent manner. Isogenic mutants of these genes were unable to grow on L-lactate-dependent medium. The hp1222 gene product functions as an NAD-independent D-LDH and also contributes to the oxidation of L-lactate; the isogenic mutant of this gene failed to grow on D-lactate-dependent medium. The parallel genes hp0140–0141 encode two nearly identical lactate permeases (LctP) that promote uptake of both D- and L-lactate. Interestingly an alternate route must also exist for lactate transport as the knockout of genes did not completely prevent growth on D- or L-lactate. Gene expression levels of hp0137–0139 and hp1222 were not enhanced by lactate as the carbon source. Expression of hp0140–0141 was slightly suppressed in the presence of L-lactate but not D-lactate. This study identified the genes contributing to the lactate utilization and demonstrated the ability of H. pylori to utilize both D- and L-lactate.
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影响因子：
3.1
作者：
Exley, RM;Goodwin, L;Tang, CM
通讯作者：
Tang, CM
DOI：
10.1073/pnas.90.12.5791
发表时间：
1993-06-15
影响因子：
11.1
作者：
COVACCI, A;CENSINI, S;RAPPUOLI, R
通讯作者：
RAPPUOLI, R
影响因子：
29.4
作者：
Lee, A;ORourke, J;Dixon, MF
通讯作者：
Dixon, MF
DOI：
10.1099/00221287-139-12-3023
发表时间：
1993-12-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
MENDZ, GL;HAZELL, SL;BURNS, BP
通讯作者：
BURNS, BP
DOI：
10.1016/0006-291x(89)91546-5
发表时间：
1989-10-31
影响因子：
3.1
作者：
DUNCAN, JD;WALLIS, JO;AZARI, MR
通讯作者：
AZARI, MR