Sheathless size-based acoustic particle separation.
Sheathless size-based acoustic particle separation.
复制标题
DOI：
10.3390/s120100905
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Zhe J
中科院分区：
文献类型：
--
作者：
Guldiken R;Jo MC;Gallant ND;Demirci U;Zhe J
颗粒分离在许多生物学和生物医学应用中具有重要意义。基于流动的方法已被用于对颗粒和细胞进行分类。然而，基于流动的颗粒分离系统的主要挑战是需要一个护套流才能成功运行。鞘液的存在稀释了分析物，需要在样品和鞘流之间进行精确的流动控制，需要复杂的设计来产生鞘流，分离效率取决于鞘液的组成。本文提出了一种利用驻表面声波进行无鞘颗粒分离的微流控平台。在这个平台中，颗粒首先在通道中心排列，而不引入任何外部护套流。然后颗粒进入第二阶段，其中颗粒被驱动到偏离中心的压力节点进行基于尺寸的分离。由于声力差异，较大的颗粒在通道中暴露于更多的横向位移。因此，不同大小的颗粒被分离到多个收集口。本发明微流控平台的突出特点是该装置不需要使用护套流来定位和对准颗粒。相反，无护套的流动聚焦和分离集成在一个单一的微流体装置，并同时完成。在本文中，我们展示了两种不同的粒径-分辨率分离；(1) 3 μm和10 μm； (2) 3 μm和5 μm。考察了输入功率、流速和颗粒浓度对分离效率的影响。这些技术具有广泛影响各个领域的潜力，包括芯片实验室系统的基本微流控元件以及集成生物和生物医学应用。
Particle separation is of great interest in many biological and biomedical applications. Flow-based methods have been used to sort particles and cells. However, the main challenge with flow based particle separation systems is the need for a sheath flow for successful operation. Existence of the sheath liquid dilutes the analyte, necessitates precise flow control between sample and sheath flow, requires a complicated design to create sheath flow and separation efficiency depends on the sheath liquid composition. In this paper, we present a microfluidic platform for sheathless particle separation using standing surface acoustic waves. In this platform, particles are first lined up at the center of the channel without introducing any external sheath flow. The particles are then entered into the second stage where particles are driven towards the off-center pressure nodes for size based separation. The larger particles are exposed to more lateral displacement in the channel due to the acoustic force differences. Consequently, different-size particles are separated into multiple collection outlets. The prominent feature of the present microfluidic platform is that the device does not require the use of the sheath flow for positioning and aligning of particles. Instead, the sheathless flow focusing and separation are integrated within a single microfluidic device and accomplished simultaneously. In this paper, we demonstrated two different particle size-resolution separations; (1) 3 μm and 10 μm and (2) 3 μm and 5 μm. Also, the effects of the input power, the flow rate, and particle concentration on the separation efficiency were investigated. These technologies have potential to impact broadly various areas including the essential microfluidic components for lab-on-a-chip system and integrated biological and biomedical applications.
登录
查看更多内容
影响因子：
44.1
作者：
Friend, James;Yeo, Leslie Y.
通讯作者：
Yeo, Leslie Y.
影响因子：
64.8
作者：
MacDonald, MP;Spalding, GC;Dholakia, K
通讯作者：
Dholakia, K
影响因子：
3.7
作者：
Moon, SangJun;Gurkan, Umut Atakan;Demirci, Utkan
通讯作者：
Demirci, Utkan
影响因子：
6.1
作者：
Kuntaegowdanahalli, Sathyakumar S.;Bhagat, Ali Asgar S.;Papautsky, Ian
通讯作者：
Papautsky, Ian
影响因子：
56.9
作者：
Huang, LR;Cox, EC;Sturm, JC
通讯作者：
Sturm, JC