Proteomic and biochemical analyses of the cotyledon and root of flooding-stressed soybean plants.
Proteomic and biochemical analyses of the cotyledon and root of flooding-stressed soybean plants.
复制标题
DOI：
10.1371/journal.pone.0065301
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Yasue H
中科院分区：
文献类型：
--
作者：
Komatsu S;Makino T;Yasue H
淹水会显著降低大豆植株的生长和籽粒产量。利用蛋白质组学和生物化学技术研究了淹水胁迫下大豆子叶和根的功能是否发生了变化。将2日龄大豆植株淹水2天，然后从根和子叶中提取蛋白质用于蛋白质组学分析。在淹水胁迫下，73和28蛋白质的丰度显着改变，分别在根和子叶。只有一种蛋白质，70 kDa的热休克蛋白（HSP 70）（Glyma17g08020.1）的积累，增加在两个器官后洪水。淹水胁迫下，子叶中HSP70蛋白丰度与生物光子发射的比值高于根中。计算机断层扫描和元素分析表明，淹水胁迫减少了子叶中草酸钙晶体的数量，表明淹水胁迫下子叶中钙离子含量增加。这些结果表明，钙离子可能通过HSP70在淹水胁迫下的子叶中发挥作用。
Flooding significantly reduces the growth and grain yield of soybean plants. Proteomic and biochemical techniques were used to determine whether the function of cotyledon and root is altered in soybean under flooding stress. Two-day-old soybean plants were flooded for 2 days, after which the proteins from root and cotyledon were extracted for proteomic analysis. In response to flooding stress, the abundance of 73 and 28 proteins was significantly altered in the root and cotyledon, respectively. The accumulation of only one protein, 70 kDa heat shock protein (HSP70) (Glyma17g08020.1), increased in both organs following flooding. The ratio of protein abundance of HSP70 and biophoton emission in the cotyledon was higher than those detected in the root under flooding stress. Computed tomography and elemental analyses revealed that flooding stress decreases the number of calcium oxalate crystal the cotyledon, indicating calcium ion was elevated in the cotyledon under flooding stress. These results suggest that calcium might play one role through HSP70 in the cotyledon under flooding stress.
登录
查看更多内容
影响因子：
2
作者：
Githiri, S. M.;Watanabe, S.;Takahashi, R.
通讯作者：
Takahashi, R.
影响因子：
4.4
作者：
Nanjo, Yohei;Skultety, Ludovit;Komatsu, Setsuko
通讯作者：
Komatsu, Setsuko
影响因子：
3.5
作者：
Bennett, M;Mehta, M;Grant, M
通讯作者：
Grant, M
影响因子：
3.3
作者：
Komatsu, Setsuko;Nanjo, Yohei;Nishimura, Minoru
通讯作者：
Nishimura, Minoru
影响因子：
4.2
作者：
Ilarslan, H;Palmer, RG;Horner, HT
通讯作者：
Horner, HT