Evidence of innervation following extracellular matrix scaffold-mediated remodelling of muscular tissues.
Evidence of innervation following extracellular matrix scaffold-mediated remodelling of muscular tissues.
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DOI：
10.1002/term.200
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发表时间：
2009-12
影响因子：
3.3
通讯作者：
Badylak, Stephen F.
中科院分区：
文献类型：
--
作者：
Agrawal, Vineet;Brown, Bryan N.;Beattie, Allison J.;Gilbert, Thomas W.;Badylak, Stephen F.
关键词：
天然猪源性细胞外基质(ECM)已成功地作为一种生物支架材料用于多种组织的定位重建。特定部位的重建过程包括支架的快速降解，同时伴随着单个核细胞、内皮细胞和骨髓来源细胞的募集，并可导致功能性骨骼和平滑肌组织的形成。然而，肌肉组织中重建支架材料的神经支配的时间和空间模式还不是很清楚。采用细胞外基质支架作为诱导性支架，对大鼠腹壁重建模型和犬食道重建模型中神经组织的存在进行了回顾性研究。在大鼠体壁模型的28天和术后91天的犬食道修复模型中，在重建的ECM中发现了成熟的神经、未成熟的神经和雪旺细胞的证据。此外，对种植在细胞外基质支架上的原代培养神经元的反应进行的显微镜和形态学研究表明，神经元的存活和生长受到细胞外基质基质的支持。最后，ECM支架快速降解产生的基质密码肽在体外以浓度依赖的方式诱导终末雪旺细胞迁移。这项研究的结果表明，使用细胞外基质组成的生物支架可以重建以神经支配为重要功能成分的组织。
Naturally occurring porcine derived extracellular matrix (ECM) has successfully been used as a biologic scaffold material for site-specific reconstruction of a wide variety of tissues. The site-specific remodeling process includes rapid degradation of the scaffold with concomitant recruitment of mononuclear cells, endothelial cells, and bone marrow derived cells, and can lead to formation of functional skeletal and smooth muscle tissue. However, the temporal and spatial patterns of innervation of the remodeling scaffold material in muscular tissues are not well understood. A retrospective study was conducted to investigate the presence of nervous tissue in a rat model of abdominal wall reconstruction and a canine model of esophageal reconstruction in which ECM scaffolds were used as inductive scaffolds. Evidence of mature nerve, immature nerve, and Schwann cells was found within the remodeled ECM at 28 days in the rat body wall model, and at 91 days post surgery in a canine model of esophageal repair. Additionally, a microscopic and morphologic study that investigated the response of primary cultured neurons seeded upon an ECM scaffold showed that neuronal survival and outgrowth was supported by the ECM substrate. Finally, matricryptic peptides resulting from rapid degradation of the ECM scaffold induced migration of terminal Schwann cells in a concentration dependent fashion in vitro. The findings of this study suggest that the reconstruction of tissues in which innervation is an important functional component is possible with use of biologic scaffolds composed of extracellular matrix.
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影响因子：
--
作者：
Gilbert, Thomas W.;Stewart-Akers, Ann M.;Woo, Savio L-Y.
通讯作者：
Woo, Savio L-Y.
DOI：
10.1002/ar.b.20079
发表时间：
2005-11-01
期刊：
Anatomical Record Part B:The New Anatomist
影响因子：
--
作者：
Carlson, Bruce M.
通讯作者：
Carlson, Bruce M.
影响因子：
2.4
作者：
Badylak, S;Meurling, S;Simmons-Byrd, A
通讯作者：
Simmons-Byrd, A
影响因子：
2.8
作者：
DIETZ, FR
通讯作者：
DIETZ, FR
DOI：
10.1115/1.2354200
发表时间：
2006-12-01
影响因子：
1.7
作者：
Gilbert, Thomas W.;Sacks, Michael S.;Chancellor, Michael B.
通讯作者：
Chancellor, Michael B.