Surface characteristics on commercial dental implants differentially activate macrophages in vitro and in vivo.
Surface characteristics on commercial dental implants differentially activate macrophages in vitro and in vivo.
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DOI：
10.1111/clr.13717
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发表时间：
2021-04
影响因子：
4.3
通讯作者：
Olivares-Navarrete R
中科院分区：
文献类型：
--
作者：
Abaricia JO;Shah AH;Ruzga MN;Olivares-Navarrete R
生物材料植入引发炎症反应，控制整合命运。M2巨噬细胞通过解决炎症期和招募祖细胞来帮助愈合来调节对植入物的反应。我们之前已经证明，改性钛（Ti）磁盘直接诱导M2巨噬细胞极化。本研究的目的是检测巨噬细胞对市售的具有相当粗糙度和不同亲水性的钛或钛合金植入物的反应。本研究对11种市售钛（A-F）或钛合金（G-K）种植体进行了研究。对每个植入物的表面形貌、化学性质和亲水性进行了表征。为了比较体外免疫反应，将人单核细胞来源的巨噬细胞植入植入物，并测量分泌的促炎性蛋白和抗炎蛋白。为了评估体内的炎症反应，我们在小鼠皮下植入了临床植入物，并用流式细胞术对植入物粘附的巨噬细胞群体进行了表征。在体外实验中，巨噬细胞在疏水植入物C上产生最高水平的促炎蛋白。相比之下，亲水植入物E产生了第二高的促炎反应。植体F和K均为亲水性，抗炎蛋白分泌量最高。同样，在体内，促炎性CD80hi巨噬细胞在植入物C和E上占主导地位，M2 CD206+巨噬细胞在植入物F和k上占主导地位。这些发现表明，仅亲水性不足以预测巨噬细胞极化的抗炎作用，其他性质（表面组成或地形）决定了免疫调节。这种体内模型可能是一种有用的筛选方法，用于比较不同几何形状或大小的临床植入物的免疫调节反应。
Biomaterial implantation provokes an inflammatory response that controls integrative fate. M2 macrophages regulate the response to implants by resolving the inflammatory phase and recruiting progenitor cells to aid healing. We have previously shown that modified titanium (Ti) disks directly induce M2 macrophage polarization. The aim of this study was to examine macrophage response to commercially available Ti or Ti alloy implants with comparable roughness and varying hydrophilicity. Eleven commercially available Ti (A-F) or Ti alloy (G-K) dental implants were examined in this study. Surface topography, chemistry, and hydrophilicity were characterized for each implant. To compare the immune response in vitro, human monocyte-derived macrophages were seeded on implants and secreted pro- and anti-inflammatory proteins measured. To evaluate the inflammatory response in vivo, mice were subcutaneously instrumented with clinical implants, and implant adherent macrophage populations were characterized by flow cytometry. Macrophages on hydrophobic Implant C produced the highest level of pro-inflammatory proteins in vitro. In contrast, hydrophilic Implant E produced the second-highest pro-inflammatory response. Implants F and K, both hydrophilic, produced the highest anti-inflammatory protein secretions. Likewise, pro-inflammatory CD80hi macrophages predominated in vivo on implants C and E, and M2 CD206+ macrophages predominated on F and K. These findings show that hydrophilicity alone is insufficient to predict the anti-inflammatory effect on macrophage polarization, and that other properties—surface composition or topography— determine immune modulation. This in vivo model may be a useful screening method to compare the immunomodulatory response to clinical implants of disparate geometry or size.
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影响因子：
--
作者：
Boyan, B D;Cheng, A;Schwartz, Z
通讯作者：
Schwartz, Z
影响因子：
4.3
作者：
Donos, N.;Hamlet, S.;Ivanovski, S.
通讯作者：
Ivanovski, S.
影响因子：
14
作者：
Abaricia, Jefferson O.;Shah, Arth H.;Olivares-Navarrete, Rene
通讯作者：
Olivares-Navarrete, Rene
影响因子：
5
作者：
Hotchkiss, Kelly M.;Sowers, Kegan T.;Olivares-Navarrete, Rene
通讯作者：
Olivares-Navarrete, Rene
影响因子：
4.3
作者：
Lotz, Ethan M.;Olivares-Navarrete, Rene;Boyan, Barbara D.
通讯作者：
Boyan, Barbara D.