Sugars promote graft union development in the heterograft of cucumber onto pumpkin.
Sugars promote graft union development in the heterograft of cucumber onto pumpkin.
复制标题
糖促进黄瓜异种移植到南瓜上的嫁接联合发育
DOI：
10.1038/s41438-021-00580-5
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发表时间：
2021-07-01
影响因子：
8.7
通讯作者：
Yu X
中科院分区：
文献类型：
--
作者：
Miao L;Li Q;Sun TS;Chai S;Wang C;Bai L;Sun M;Li Y;Qin X;Zhang Z;Yu X
异种嫁接被广泛应用于几种重要的经济作物的生产，但嫁接结合形成的分子机制尚不清楚。本研究以黄瓜嫁接到南瓜上，研究了嫁接体的结合发育，并对嫁接界面基因的全基因组时空表达进行了全面研究。组织学分析表明，砧木在韧皮部和木质部重新连接后恢复生长，嫁接后3 d接穗产生的愈伤组织较砧木明显。转录组数据一致地揭示了接穗和砧木在血管连接和愈合过程中与形成层发育、细胞周期和糖代谢相关的基因表达的特异性反应，表明了不同的机制。此外，在血管重新连接过程中，移植物连接处的糖水平降低，糖酶活性显著改变。接下来，我们发现外源葡萄糖显著延缓了嫁接黄化苗的愈合过程，并促进了嫁接成功、木质部重连和嫁接植株的生长。这表明嫁接结合的形成需要正确的糖含量。此外，我们还发现，以雷帕霉素（TOR）抑制剂AZD-8055为靶点，能以较低的能量电荷延迟嫁接结合的形成，并且外源葡萄糖处理AZD-8055能增强嫁接植株的木质部重连接和生长。综上所述，我们的研究结果表明，糖通过促进黄瓜/南瓜的生长，在移植物愈合中发挥了积极的作用，为了解移植物愈合和未来异种嫁接的应用提供了有用的信息。
The use of heterografts is widely applied for the production of several important commercial crops, but the molecular mechanism of graft union formation remains poorly understood. Here, cucumber grafted onto pumpkin was used to study graft union development, and genome-wide tempo-spatial gene expression at the graft interface was comprehensively investigated. Histological analysis suggested that resumption of the rootstock growth occurred after both phloem and xylem reconnection, and the scion showed evident callus production compared with the rootstock 3 days after grafting. Consistently, transcriptome data revealed specific responses between the scion and rootstock in the expression of genes related to cambium development, the cell cycle, and sugar metabolism during both vascular reconnection and healing, indicating distinct mechanisms. Additionally, lower levels of sugars and significantly changed sugar enzyme activities at the graft junction were observed during vascular reconnection. Next, we found that the healing process of grafted etiolated seedlings was significantly delayed, and graft success, xylem reconnection, and the growth of grafted plants were enhanced by exogenous glucose. This demonstrates that graft union formation requires the correct sugar content. Furthermore, we also found that graft union formation was delayed with a lower energy charge by the target of rapamycin (TOR) inhibitor AZD-8055, and xylem reconnection and the growth of grafted plants were enhanced under AZD-8055 with exogenous glucose treatment. Taken together, our results reveal that sugars play a positive role in graft union formation by promoting the growth of cucumber/pumpkin and provide useful information for understanding graft union healing and the application of heterografting in the future.
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影响因子：
6.5
作者：
Hugo Diaz-Granados, Victor;Manuel Lopez-Lopez, Jorge;Lara-Nunez, Aurora
通讯作者：
Lara-Nunez, Aurora
影响因子：
4.3
作者：
Hu, Li-Ping;Meng, Fan-Zhen;Zhang, Zhen-Xian
通讯作者：
Zhang, Zhen-Xian
高通量测序对葫芦和南瓜砧木西瓜嫁接反应 mRNA 的全基因组鉴定和比较分析
影响因子：
3.1
作者：
Liu, Na;Yang, Jinghua;Zhang, Mingfang
通讯作者：
Zhang, Mingfang
影响因子：
4.8
作者：
Flaishman, Moshe A.;Loginovsky, Kamelia;Lev-Yadun, Simcha
通讯作者：
Lev-Yadun, Simcha
影响因子：
4.3
作者：
ALONI, R
通讯作者：
ALONI, R