TaCPK2-A, a calcium-dependent protein kinase gene that is required for wheat powdery mildew resistance enhances bacterial blight resistance in transgenic rice.
TaCPK2-A, a calcium-dependent protein kinase gene that is required for wheat powdery mildew resistance enhances bacterial blight resistance in transgenic rice.
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TaCPK2-A 是小麦白粉病抗性所需的钙依赖性蛋白激酶基因，可增强转基因水稻的白叶枯病抗性
DOI：
10.1093/jxb/ert146
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发表时间：
2013-08
影响因子：
6.9
通讯作者：
Mao L
中科院分区：
文献类型：
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作者：
Geng S;Li A;Tang L;Yin L;Wu L;Lei C;Guo X;Zhang X;Jiang G;Zhai W;Wei Y;Zheng Y;Lan X;Mao L
钙依赖性蛋白激酶（CPKs）是参与复杂的免疫和应激信号网络的重要钙信号组分，但CPK基因在六倍体小麦中的功能研究还很有限。以前，TaCPK 2被证明是由小麦中的白粉病（Blumeria graminis triumphis，Bgt）感染诱导的。在这里，其在抗病性中的功能进一步表征。本研究表明在A亚基因组同源物（TaCPK 2-A）和D亚基因组同源物（TaCPK 2-D）的启动子上分别存在防御反应顺式元件和冷反应顺式元件。然后使用基因组特异性引物通过定量实时PCR（qRT-PCR）确认它们的表达模式，其中TaCPK 2-A被Bgt处理诱导，而TaCPK 2-D主要响应于冷处理。TaCPK 2-A通过病毒诱导的基因沉默（VIGS）下调导致抗Bgt小麦品系的抗性丧失，表明TaCPK 2-A是白粉病抗性所必需的。此外，TaCPK 2-A在水稻中的过表达增强了细菌性枯萎病（Xanthomonasspleenpv.）Xoo）抗性。qRT-PCR分析表明，TaCPK 2-A在水稻中的过表达促进了OsWRKY 45 -1的表达，OsWRKY 45 -1是一种转录因子，通过调控茉莉酸和水杨酸信号转导基因参与水稻的抗真菌和抗细菌。在小麦TaCPK 2-AVIGS植物中发现了相反的效果，其中OsWRKY 45 -1的同源物被显著抑制。这些数据表明，WRKY 45 -1和相关的防御反应基因的CPK 2基因的调制可能是常见的机制，在禾本科植物的多重疾病的抗性，这可能已经经历了亚功能化的启动子形成六倍体小麦之前。
Calcium-dependent protein kinases (CPKs) are important Ca2+ signalling components involved in complex immune and stress signalling networks; but the knowledge of CPK gene functions in the hexaploid wheat is limited. Previously, TaCPK2 was shown to be inducible by powdery mildew (Blumeria graminis tritici, Bgt) infection in wheat. Here, its functions in disease resistance are characterized further. This study shows the presence of defence-response and cold-response cis-elements on the promoters of the A subgenome homoeologue (TaCPK2-A) and D subgenome homoeologue (TaCPK2-D), respectively. Their expression patterns were then confirmed by quantitative real-time PCR (qRT-PCR) using genome-specific primers, where TaCPK2-A was induced by Bgt treatment while TaCPK2-D mainly responded to cold treatment. Downregulation of TaCPK2-A by virus-induced gene silencing (VIGS) causes loss of resistance to Bgt in resistant wheat lines, indicating that TaCPK2-A is required for powdery mildew resistance. Furthermore, overexpression of TaCPK2-A in rice enhanced bacterial blight (Xanthomonas oryzae pv. oryzae, Xoo) resistance. qRT-PCR analysis showed that overexpression of TaCPK2-A in rice promoted the expression of OsWRKY45-1, a transcription factor involved in both fungal and bacterial resistance by regulating jasmonic acid and salicylic acid signalling genes. The opposite effect was found in wheat TaCPK2-A VIGS plants, where the homologue of OsWRKY45-1 was significantly repressed. These data suggest that modulation of WRKY45-1 and associated defence-response genes by CPK2 genes may be the common mechanism for multiple disease resistance in grass species, which may have undergone subfunctionalization in promoters before the formation of hexaploid wheat.
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影响因子：
20.5
作者：
Boudsocq, Marie;Sheen, Jen
通讯作者：
Sheen, Jen
小麦 CDPK 基因家族的进化和功能研究 (Triticum aestivum L.)
影响因子：
5.1
作者：
Li, Ai-Li;Zhu, Yuan-Fang;Mao, Long
通讯作者：
Mao, Long
影响因子：
2.7
作者：
Li, AL;Wang, ML;Jia, JZ
通讯作者：
Jia, JZ
DOI：
10.1073/pnas.0502954102
发表时间：
2005-07-26
影响因子：
11.1
作者：
Ludwig, AA;Saitoh, H;Romeis, T
通讯作者：
Romeis, T
影响因子：
64.8
作者：
通讯作者：
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