Detection of local and remote cellular damage caused by spinal cord and peripheral nerve injury using a heat shock signaling reporter system.
Detection of local and remote cellular damage caused by spinal cord and peripheral nerve injury using a heat shock signaling reporter system.
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DOI：
10.1016/j.ibror.2018.11.003
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发表时间：
2018-12
期刊：
影响因子：
2.6
通讯作者：
Torii M
中科院分区：
文献类型：
--
作者：
Hashimoto-Torii K;Sasaki M;Chang YW;Hwang H;Waxman SG;Kocsis JD;Rakic P;Torii M
HSE-RFP报告基因检测脊髓损伤中的原发性和继发性受损神经细胞。HSE-RFP报告基因检测坐骨神经损伤中的原发性和继发性受损神经细胞。报告基因阳性的二次损伤神经元显示出改变的生理特性。脊髓和周围神经损伤导致对局部损伤细胞以及与它们功能性连接的远端细胞的广泛损伤。原发性和继发性损伤均可引起广泛的临床异常，包括神经性疼痛以及认知和记忆功能障碍。然而，这些异常的机制仍然不清楚，等待新的方法来识别受影响的细胞，以检查其分子，细胞和生理特征。在这里，我们报告说，原发性和继发性损伤的脊髓和周围神经损伤的小鼠模型中的细胞可以在体内检测使用一种新的荧光报告系统的基础上，通过激活热休克因子1的即时应激反应。我们还提供了证据，改变的电生理特性的神经元阳性继发性损伤。全面鉴定损伤的，但存活的细胞位于接近和远离损伤部位的位置在体内将提供一种方法来研究其病理生理学和可能的预防其进一步恶化。
The HSE-RFP reporter detects primarily- and secondarily-damaged neural cells in spinal cord injury. The HSE-RFP reporter detects primarily- and secondarily-damaged neural cells in sciatic nerve injury. Reporter-positive secondarily-injured neurons show altered physiological properties. Spinal cord and peripheral nerve injury results in extensive damage to the locally injured cells as well as distant cells that are functionally connected to them. Both primary and secondary damage can cause a broad range of clinical abnormalities, including neuropathic pain and cognitive and memory dysfunction. However, the mechanisms underlying these abnormalities remain unclear, awaiting new methods to identify affected cells to enable examination of their molecular, cellular and physiological characteristics. Here, we report that both primary and secondary damage to cells in mouse models of spinal cord and peripheral nerve injury can be detected in vivo using a novel fluorescent reporter system based on the immediate stress response via activation of Heat Shock Factor 1. We also provide evidence for altered electrophysiological properties of reporter-positive secondarily-injured neurons. The comprehensive identification of injured, but surviving cells located both close and at distant locations from the injury site in vivo will provide a way to study their pathophysiology and possibly prevention of their further deterioration.
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影响因子：
9.3
作者：
Dubový P;Brázda V;Klusáková I;Hradilová-Svíženská I
通讯作者：
Hradilová-Svíženská I
影响因子：
4.6
作者：
Knerlich-Lukoschus F;Held-Feindt J
通讯作者：
Held-Feindt J
影响因子：
9.2
作者：
Erta M;Quintana A;Hidalgo J
通讯作者：
Hidalgo J
影响因子：
3.4
作者：
Kang, Soo Kyung;So, Hyeun Hwa;Kim, Cheul Hong
通讯作者：
Kim, Cheul Hong
影响因子：
13.9
作者：
Costigan M;Scholz J;Woolf CJ
通讯作者：
Woolf CJ