Detection of malaria sporozoites expelled during mosquito sugar feeding.
Detection of malaria sporozoites expelled during mosquito sugar feeding.
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DOI：
10.1038/s41598-018-26010-6
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发表时间：
2018-05-15
影响因子：
4.6
通讯作者：
Walker T
中科院分区：
文献类型：
--
作者：
Brugman VA;Kristan M;Gibbins MP;Angrisano F;Sala KA;Dessens JT;Blagborough AM;Walker T
疟疾是一种全球性的严重疾病，由按蚊属的蚊子传播。迅速发现能够传播疟疾的传染性蚊子的能力对于监测、控制和消灭工作至关重要。目前的方法主要依赖于大规模的蚊子收集，然后是劳动密集型的唾液腺解剖或酶联免疫吸附（ELISA）方法来检测子孢子。使用强迫流涎，我们在这里证明，按蚊感染疟原虫驱逐子孢子在糖喂养。排出的子孢子可以在两种糖浸泡的基质上检测到，棉花和Whatman FTA卡，子孢子DNA可以使用实时PCR检测。这些结果表明，一个简单，快速的方法来检测感染性蚊子孢子的存在，并强调潜在的实验室应用调查蚊子疟疾的相互作用。我们的研究结果表明，FTA卡可以作为一种简单，有效和经济的工具，在加强疟疾的现场监测活动。
Malaria is a severe disease of global importance transmitted by mosquitoes of the genus Anopheles. The ability to rapidly detect the presence of infectious mosquitoes able to transmit malaria is of vital importance for surveillance, control and elimination efforts. Current methods principally rely on large-scale mosquito collections followed by labour-intensive salivary gland dissections or enzyme-linked immunosorbent (ELISA) methods to detect sporozoites. Using forced salivation, we demonstrate here that Anopheles mosquitoes infected with Plasmodium expel sporozoites during sugar feeding. Expelled sporozoites can be detected on two sugar-soaked substrates, cotton wool and Whatman FTA cards, and sporozoite DNA is detectable using real-time PCR. These results demonstrate a simple and rapid methodology for detecting the presence of infectious mosquitoes with sporozoites and highlight potential laboratory applications for investigating mosquito-malaria interactions. Our results indicate that FTA cards could be used as a simple, effective and economical tool in enhancing field surveillance activities for malaria.
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影响因子：
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作者：
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