Three dimensional mapping of neuropeptides and lipids in crustacean brain by mass spectral imaging.
Three dimensional mapping of neuropeptides and lipids in crustacean brain by mass spectral imaging.
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DOI：
10.1016/j.jasms.2009.01.017
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发表时间：
2009-06
影响因子：
3.2
通讯作者：
Li L
中科院分区：
文献类型：
--
作者：
Chen R;Hui L;Sturm RM;Li L
成像质谱作为一种强有力的工具，已被广泛应用于生物组织中蛋白质、肽、药物化合物、代谢物和脂质的定位。本文应用三维质谱成像技术研究了多种神经肽家族和脂类在北方蟹脑中的分布规律。不同的基质/溶剂组合进行了比较，优先提取和检测的神经肽和脂质。结合形态学信息，使用基质辅助激光解吸/电离串联飞行时间质谱法（MALDI-TOF/TOF MS）确定了许多神经肽在整个大脑三维结构中的分布。不同的定位模式，观察到不同的神经肽家族，并显示独特的分布模式，从共同的家庭分布趋势不同的亚型也被检测到。此外，从脑组织切片中鉴定并绘制了多种脂质。为了确认它们的身份，进行MS/MS裂解。不同的脂质种类显示出不同的定位模式，这表明它们在神经系统中的潜在不同的功能作用。
Imaging mass spectrometry is emerging as a powerful tool that has been applied extensively for the localization of proteins, peptides, pharmaceutical compounds, metabolites, and lipids in biological tissues. In this paper, three dimensional mass spectral imaging (3D MSI) technique was developed to examine distribution patterns of multiple neuropeptide families and lipids in the brain of the crab Cancer borealis. Different matrix/solvent combinations were compared for preferential extraction and detection of neuropeptides and lipids. Combined with morphological information, the distribution of numerous neuropeptides throughout the 3D structure of brain was determined using matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF MS). Different localization patterns were observed for different neuropeptide families, and isoforms displaying unique distribution patterns that were distinct from the common family distribution trends were also detected. In addition, multiple lipids were identified and mapped from brain tissue slices. To confirm their identities, MS/MS fragmentation was performed. Different lipid species displayed distinct localization patterns, suggesting their potential different functional roles in the nervous system.
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影响因子：
48
作者：
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通讯作者：
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影响因子：
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通讯作者：
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