Films of agarose enable rapid formation of giant liposomes in solutions of physiologic ionic strength.
Films of agarose enable rapid formation of giant liposomes in solutions of physiologic ionic strength.
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DOI：
10.1021/ja805625u
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发表时间：
2009-02-11
影响因子：
15
通讯作者：
Mayer, Michael
中科院分区：
文献类型：
--
作者：
Horger, Kim S.;Estes, Daniel J.;Capone, Ricardo;Mayer, Michael
本文描述了一种在生理离子强度的溶液如磷酸盐缓冲盐水（PBS）或150 mM KCl中形成巨型脂质体的方法。这些细胞大小的脂质体的形成从部分干燥的琼脂糖和脂质的混合膜进行。在盐水溶液中水合琼脂糖和脂质的膜导致混合膜的溶胀和部分溶解，并伴随着高产率的巨大脂质体的快速形成。这种方法不需要电场或特殊脂质的存在;它从纯磷脂酰胆碱脂质或含有胆固醇或带负电荷的脂质的脂质混合物中产生巨大的脂质体。琼脂糖和脂质的混合膜甚至能够在PBS中由脂质组合物形成巨大的脂质体，所述脂质组合物通常对于脂质体形成是有问题的，例如纯磷脂酰丝氨酸、纯磷脂酰甘油和asolectin。本文讨论了生物物理方面的形成巨大的脂质体从混合膜的琼脂糖和脂质相比，已建立的方法，并表明，温和的水合混合膜的琼脂糖和脂质是一个简单的，快速的，可重复的程序，以产生巨大的脂质体的各种脂质组合物的生理离子强度的解决方案，而不需要专门的设备。
This paper describes a method to form giant liposomes in solutions of physiologic ionic strength, such as phosphate buffered saline (PBS) or 150 mM KCl. Formation of these cell-sized liposomes proceeded from hybrid films of partially dried agarose and lipids. Hydrating the films of agarose and lipids in aqueous salt solutions resulted in swelling and partial dissolution of the hybrid films and in concomitant rapid formation of giant liposomes in high yield. This method did not require the presence of an electric field or specialized lipids; it generated giant liposomes from pure phosphatidylcholine lipids or from lipid mixtures that contained cholesterol or negatively charged lipids. Hybrid films of agarose and lipids even enabled the formation of giant liposomes in PBS from lipid compositions that are typically problematic for liposome formation, such as pure phosphatidylserine, pure phosphatidylglycerol, and asolectin. This paper discusses biophysical aspects of the formation of giant liposomes from hybrid films of agarose and lipids in comparison to established methods and shows that gentle hydration of hybrid films of agarose and lipids is a simple, rapid, and reproducible procedure to generate giant liposomes of various lipid compositions in solutions of physiologic ionic strength without the need for specialized equipment.
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影响因子：
5.8
作者：
Castile, JD;Taylor, KMG
通讯作者：
Taylor, KMG
DOI：
10.1081/bio-120025410
发表时间：
2003-01-01
期刊：
ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
影响因子：
--
作者：
Constantinescu, I;Levin, E;Gyongyossy-Issa, M
通讯作者：
Gyongyossy-Issa, M
影响因子：
3.4
作者：
FARGE, E;DEVAUX, PF
通讯作者：
DEVAUX, PF
影响因子：
56.9
作者：
ISRAELACHVILI, JN;MCGUIGGAN, PM
通讯作者：
MCGUIGGAN, PM
影响因子：
--
作者：
Itagaki, H;Fukiishi, H;Watase, M
通讯作者：
Watase, M