In vivo microelectrode monitoring of real-time hydrogen concentration in different tissues of rats after inhaling hydrogen gas.
In vivo microelectrode monitoring of real-time hydrogen concentration in different tissues of rats after inhaling hydrogen gas.
复制标题
体内微电极实时监测大鼠吸入氢气后不同组织的氢气浓度
DOI：
10.4103/2045-9912.330694
复制
发表时间：
2022-07
影响因子：
2.9
通讯作者：
Qin SC
中科院分区：
文献类型：
--
作者：
Liu BY;Xue JL;Gu QQ;Zhao M;Zhang MY;Wang MY;Wang Y;Qin SC
多项研究报道了氢气的医学效应。由于摄入后难以测量体内氢气浓度，且氢气具有高爆炸风险，因此关于氢气剂量 - 反应关系及组织浓度的研究较少。在此，我们首次使用电化学传感器，监测了吸入不同浓度氢气（4%、42%和67%）后，大鼠不同组织（包括脑、肝、脾、肾、大腿肌肉、腹股沟白色脂肪组织和性腺白色脂肪组织）中的实时氢气浓度。同一组织中的氢气浓度呈现出剂量依赖性反应。平衡浓度值在脑中最高，在大腿肌肉中最低。与其他组织相比，大腿肌肉和白色脂肪组织中的饱和与去饱和曲线变化更为缓慢。这些结果为基础研究和临床试验中氢气剂量的选择提供了基础信息。本实验于2020年3月18日获得山东第一医科大学暨山东省医学科学院实验动物伦理委员会批准（编号2020 - 1028）。
Medical effects of hydrogen have been reported in many studies. Due to difficulties in measuring hydrogen concentration in vivo after intake and high explosive risks of hydrogen, studies about dose-response relationships and tissue concentrations of hydrogen are few. Here, for the first time, we monitored real-time hydrogen concentrations in different tissues in rats including brain, liver, spleen, kidney, thigh muscle, inguinal white adipose tissue, and gonadal white adipose tissue after inhaling different concentrations of hydrogen (4%, 42%, and 67%) using an electrochemical sensor. Hydrogen concentrations in the same tissue showed a dose-dependent response. The equilibrium concentration values were highest in the brain and lowest in the thigh muscle. The saturation and desaturation curves changed more slowly in the thigh muscle and white adipose tissues than in other tissues. These results provide fundamental information for the selection of hydrogen dose applications in basic research and clinical trials. The experiments were approved by the Laboratory Animal Ethics Committee of Shandong First Medical University & Shandong Academy of Medical Sciences (No. 2020-1028) on March 18, 2020.
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影响因子：
2.9
作者：
Hu HW;Chen ZG;Liu JG;Chen G
通讯作者：
Chen G
吸入氢气可减轻代谢综合征大鼠的非酒精性脂肪肝
影响因子：
3.4
作者：
Liu, Boyan;Xue, Junli;Qin, Shucun
通讯作者：
Qin, Shucun
氢气影响高密度脂蛋白相关酶并降低高脂饮食大鼠的氧化磷脂水平
影响因子：
6.1
作者：
Liu, Boyan;Zhao, Min;Qin, Shucun
通讯作者：
Qin, Shucun
DOI：
10.1016/j.bbrc.2008.05.165
发表时间：
2008-08-15
影响因子：
3.1
作者：
Hayashida, Kentaro;Sano, Motoaki;Fukuda, Keiichi
通讯作者：
Fukuda, Keiichi
影响因子：
9
作者：
Carcassi, MN;Fineschi, F
通讯作者：
Fineschi, F