Chemical factors induce aggregative multicellularity in a close unicellular relative of animals.
Chemical factors induce aggregative multicellularity in a close unicellular relative of animals.
复制标题
DOI：
10.1073/pnas.2216668120
复制
发表时间：
2023-05-02
影响因子：
11.1
通讯作者：
Gerdt, Joseph P.
中科院分区：
文献类型：
--
作者：
Ros-Rocher, Nuria;Kidner, Ria Q.;Gerdt, Catherine;Davidson, W. Sean;Ruiz-Trillo, Inaki;Gerdt, Joseph P.
第一批多细胞动物是否利用化学线索来调节细胞聚集仍然缺乏研究。我们发现，在一些动物和其他单细胞物种中观察到，单细胞近亲动物的聚集是由化学线索诱导的。这一发现扩大了化学调节的多细胞在单细胞动物近亲中的流行程度，这表明这种特征可能在动物的近亲单细胞祖先中很常见。我们的研究结果还提供了一种生化工具，可以进一步剖析新兴单细胞模型中的聚集阶段，并确定它们与动物聚集机制的同源程度。在许多动物组织中，受调节的细胞聚集是发育和愈合的重要过程。在一些动物和一些近亲单细胞物种中，细胞聚集是由扩散的化学信号调节的。然而，目前尚不清楚受调控的细胞聚集是否是第一批多细胞动物和/或它们的单细胞祖先生命周期的一部分。为了填补这一空白，我们研究了动物最接近的单细胞亲戚之一的细胞聚集的触发因素-丝状海capaspora owczarzaki。我们发现Capsaspora聚集是由化学线索诱导的，正如在一些最早的分支动物和其他单细胞物种中观察到的那样。具体来说，我们发现存在于脂蛋白中的钙离子和脂质共同作用，诱导有活力的Capsaspora细胞聚集。我们还发现这个多细胞阶段是可逆的，因为线索的耗尽触发分解，这可以在再诱导时克服。我们的发现表明，化学调节的聚集在全息动物分支的不同成员中是重要的。因此，这种表型似乎是动物单细胞祖先生命周期的一部分。
Whether the first multicellular animals utilized chemical cues to regulate cellular aggregation remains poorly studied. We discovered that aggregation of a unicellular close animal relative is induced by chemical cues, as observed in some animals and other unicellular species. This discovery expands the prevalence of chemically regulated multicellularity in unicellular animal relatives, which suggests that this trait may have been common in the close unicellular ancestors of animals. Our findings also yield a biochemical tool to further dissect the stages of aggregation in an emerging unicellular model and determine the extent of their homology to aggregation mechanisms in animals. Regulated cellular aggregation is an essential process for development and healing in many animal tissues. In some animals and a few distantly related unicellular species, cellular aggregation is regulated by diffusible chemical cues. However, it is unclear whether regulated cellular aggregation was part of the life cycles of the first multicellular animals and/or their unicellular ancestors. To fill this gap, we investigated the triggers of cellular aggregation in one of animals’ closest unicellular living relatives—the filasterean Capsaspora owczarzaki. We discovered that Capsaspora aggregation is induced by chemical cues, as observed in some of the earliest branching animals and other unicellular species. Specifically, we found that calcium ions and lipids present in lipoproteins function together to induce aggregation of viable Capsaspora cells. We also found that this multicellular stage is reversible as depletion of the cues triggers disaggregation, which can be overcome upon reinduction. Our finding demonstrates that chemically regulated aggregation is important across diverse members of the holozoan clade. Therefore, this phenotype was plausibly integral to the life cycles of the unicellular ancestors of animals.
登录
查看更多内容
影响因子：
19
作者：
Goldstein, Bob;King, Nicole
通讯作者：
King, Nicole
影响因子：
1.5
作者：
Costa, Manoel L.;Rosa, Ivone de Andrade;Coutinho, Cristiano C.
通讯作者：
Coutinho, Cristiano C.
对真核生物的分类，命名法和多样性进行修订。
DOI：
10.1111/jeu.12691
发表时间：
2019-01
期刊：
The Journal of eukaryotic microbiology
影响因子：
--
作者：
Adl SM;Bass D;Lane CE;Lukeš J;Schoch CL;Smirnov A;Agatha S;Berney C;Brown MW;Burki F;Cárdenas P;Čepička I;Chistyakova L;Del Campo J;Dunthorn M;Edvardsen B;Eglit Y;Guillou L;Hampl V;Heiss AA;Hoppenrath M;James TY;Karnkowska A;Karpov S;Kim E;Kolisko M;Kudryavtsev A;Lahr DJG;Lara E;Le Gall L;Lynn DH;Mann DG;Massana R;Mitchell EAD;Morrow C;Park JS;Pawlowski JW;Powell MJ;Richter DJ;Rueckert S;Shadwick L;Shimano S;Spiegel FW;Torruella G;Youssef N;Zlatogursky V;Zhang Q
通讯作者：
Zhang Q
影响因子：
2.5
作者：
Brown, Matthew W.;Silberman, Jeffrey D.;Spiegel, Frederick W.
通讯作者：
Spiegel, Frederick W.
影响因子：
9.2
作者：
Brown, Matthew W.;Kolisko, Martin;Roger, Andrew J.
通讯作者：
Roger, Andrew J.