An amphiphilic degradable polymer/hydroxyapatite composite with enhanced handling characteristics promotes osteogenic gene expression in bone marrow stromal cells.
An amphiphilic degradable polymer/hydroxyapatite composite with enhanced handling characteristics promotes osteogenic gene expression in bone marrow stromal cells.
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DOI：
10.1016/j.actbio.2013.06.013
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发表时间：
2013-09
影响因子：
9.7
通讯作者：
Song, Jie
中科院分区：
文献类型：
--
作者：
Kutikov, Artem B.;Song, Jie
静电纺聚合物/羟基磷灰石（HA）复合材料结合了生物可降解性和骨传导性，对于骨骼组织工程应用具有吸引力。然而，大多数生物可降解聚合物（例如 PLA）都是疏水性的，并且与 HA 的界面粘合力不充分，从而损害了复合材料的结构均匀性、机械完整性和生物性能。为了克服这一挑战，我们在聚（D,L-乳酸）中加入了亲水性聚乙二醇（PEG）嵌段，以提高可降解聚合物与 HA 的粘附力。两亲性三嵌段共聚物PLA-PEG-PLA (PELA)提高了HA含量为25 wt%的HA-PELA悬浮液的稳定性，该悬浮液很容易电纺成纤维尺寸均匀的HA-PELA复合支架。 HA-PELA 具有高度可延展性（失效应变 >200%，HA-PLA 为 <40%）、超亲水性（~0° 水接触角，HA-PLA >100°），并且由于 HA 和 PEG 之间有利的相互作用，在水合后储能模量增加了 8 倍（与 HA-PLA 的退化不同）。 HA-PELA 还可以更好地促进未刺激培养物中骨髓基质细胞的骨软骨谱系定型，并且在诱导后支持比 HA-PLA 更有效的成骨作用。我们证明，化学掺入 PEG 是提高骨组织工程应用中可降解聚合物/HA 复合材料性能的有效策略。
Electrospun polymer/hydroxyapatite (HA) composites combining biodegradability with osteoconductivity are attractive for skeletal tissue engineering applications. However, most biodegradable polymers such as PLA are hydrophobic and do not blend with adequate interfacial adhesion with HA, compromising the structural homogeneity, mechanical integrity, and biological performance of the composite. To overcome this challenge, we incorporated a hydrophilic polyethylene glycol (PEG) block to poly(D,L-lactic acid) to improve the adhesion of the degradable polymer with HA. The amphiphilic triblock copolymer PLA-PEG-PLA (PELA) improved the stability of HA-PELA suspension at 25 wt% HA content, which was readily electrospun into HA-PELA composite scaffolds with uniform fiber dimensions. HA-PELA was highly extensible (failure strain >200% vs. <40% for HA-PLA), superhydrophilic (~0° water contact angle vs. >100° for HA-PLA), and exhibited an 8-fold storage modulus increase (unlike deterioration for HA-PLA) upon hydration, owing to the favorable interaction between HA and PEG. HA-PELA also better promoted osteochondral lineage commitment of bone marrow stromal cells in unstimulated culture and supported far more potent osteogenesis upon induction than HA-PLA. We demonstrate that the chemical incorporation of PEG is an effective strategy to improve the performance of degradable polymer/HA composites for bone tissue engineering applications.
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影响因子：
14
作者：
Kim, SS;Park, MS;Kim, BS
通讯作者：
Kim, BS
影响因子：
4.9
作者：
Kim, Hae-Won;Lee, Hae-Hyoung;Knowles, J. C.
通讯作者：
Knowles, J. C.
影响因子：
3.9
作者：
Lian, JB;Stein, GS;van Wijnen, AJ
通讯作者：
van Wijnen, AJ
影响因子：
--
作者：
Lieb, E;Tessmar, J;Schulz, MB
通讯作者：
Schulz, MB
影响因子：
2.7
作者：
Filion, Tera M.;Kutikov, Artem;Song, Jie
通讯作者：
Song, Jie