Immunohistochemical and ultrastructural characteristics of interstitial cells of Cajal in the rabbit duodenum. Presence of a single cilium.
Immunohistochemical and ultrastructural characteristics of interstitial cells of Cajal in the rabbit duodenum. Presence of a single cilium.
复制标题
DOI：
10.1111/j.1582-4934.2007.00064.x
复制
发表时间：
2007-07
影响因子：
5.3
通讯作者：
Junquera SR
中科院分区：
文献类型：
--
作者：
Junquera C;Martínez-Ciriano C;Castiella T;Serrano P;Azanza MJ;Junquera SR
Santiago Ramón y Cajal发现了一种与肌丛和肠环形肌层的平滑肌细胞有关的新型细胞。根据它们的形态、关系和染色特征，他认为这些细胞是原始神经元。一个世纪后，尽管细胞生物学取得了重大进展，但对于许多研究人员来说，间质细胞（ICCs）仍然存在争议。本研究的目的是对兔十二指肠ICCs进行免疫组化和超微结构表征。我们发现间质细胞c-Kit、CD34和nestin呈阳性，Ki67蛋白也呈阳性，与体细胞增殖密切相关。通过电子显微镜，我们描述了肠神经节周围的icc。它们呈三角形或纺锤形，细胞核非常大，核内染色质很少，核周细胞质周围有一层薄的胞质，胞质突起长。ICC突在平滑肌细胞间渗透，并与位于圆形肌层结缔组织的其他ICC突偶联，形成三维网络。通过间隙样连接的细胞间接触是频繁的。ICCs也与平滑肌细胞建立间隙样连接。我们还观察到，在圆形肌肉层环绕肌束的结缔组织中，通常靠近神经干，存在星状形态的间质细胞群。这些细胞与周围的肌肉细胞建立不同类型的联系。此外，在ICC中首次发现单根纤毛呈9 + 0结构。总之，我们报告c-kit、CD34、nestin和ki67阳性染色。icc符合通常的透射电子显微镜（TEM）标准。至少在一些ICCs中显示了一个新的超微结构特征：单个纤毛的存在。兔十二指肠内的某些ICCs群体具有祖细胞中常见的免疫组织化学和超微结构特征。
Santiago Ramón y Cajal discovered a new type of cell related to the myenteric plexus and also to the smooth muscle cells of the circular muscle layer of the intestine. Based on their morphology, relationships and staining characteristics, he considered these cells as primitive neurons. One century later, despite major improvements in cell biology, the interstitial cells of Cajal (ICCs) are still controversial for many researchers. The aim of study was to perform an immunohistochemical and ultrastructural characterization of the ICCs in the rabbit duo-denum. We have found interstitial cells that are positive for c-Kit, CD34 and nestin and are also positive for Ki67 protein, tightly associated with somatic cell proliferation. By means of electron microscopy, we describe ICCs around enteric ganglia. They present triangular or spindle forms and a very voluminous nucleus with scarce per-inuclear chromatin surrounded by a thin perinuclear cytoplasm that expands with long cytoplasmic processes. ICC processes penetrate among the smooth muscle cells and couple with the processes of other ICCs located in the connective tissue of the circular muscle layer and establish a three-dimensional network. Intercellular con-tacts by means of gap-like junctions are frequent. ICCs also establish gap-like junctions with smooth muscle cells. We also observe a population of interstitial cells of stellate morphology in the connective tissue that sur-rounds the muscle bundles in the circular muscle layer, usually close to nervous trunks. These cells establish different types of contacts with the muscle cells around them. In addition, the presence of a single cilium show-ing a structure 9 + 0 in an ICC is demonstrated for the first time. In conclusion, we report positive staining c-kit, CD34, nestin and Ki 67. ICCs fulfilled the usual transmission electron microscopy (TEM) criteria. A new ultrastructural characteristic of at least some ICCs is demonstrated: the presence of a single cilium. Some populations of ICCs in the rabbit duodenum present certain immunohistochemical and ultrastructural characteristics that often are present in progenitor cells.
登录
查看更多内容
影响因子：
6.6
作者：
Davidson, RA;McCloskey, KD
通讯作者：
McCloskey, KD
影响因子：
2
作者：
Martinez-Ciriano, C;Junquera, C;Blasco, J
通讯作者：
Blasco, J
DOI：
10.1016/0736-5748(87)90047-5
发表时间：
1987-01-01
影响因子：
1.8
作者：
COHEN, E;MEININGER, V
通讯作者：
MEININGER, V
影响因子：
2.5
作者：
Almazán, G;Vela, JM;Guaza, C
通讯作者：
Guaza, C
DOI：
10.1111/j.1582-4934.2005.tb00387.x
发表时间：
2005-10-01
影响因子：
5.3
作者：
Gherghiceanu, M;Popescu, LM
通讯作者：
Popescu, LM