Drosophila attack inhibits hyphal regeneration and defense mechanisms activation for the fungus Trichoderma atroviride

Drosophila attack inhibits hyphal regeneration and defense mechanisms activation for the fungus Trichoderma atroviride
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果蝇攻击抑制真菌深绿木霉的菌丝再生和防御机制激活

DOI:
10.1038/s41396-021-01068-9
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发表时间:
2021
期刊:
The ISME Journal
影响因子:
--
通讯作者:
Atriztán-Hernández K
Atriztán-Hernández K
中科院分区:
--
文献类型:
--
作者:
Atriztán-Hernández K

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对伤害作出反应的能力是不同王国的生物体共有的一个过程,它可以导致整个身体部位的再生或失去结构或器官。丝状真菌构成了丰富的食物来源,确保其捕食者的生存和繁殖,因此不断受到机械损伤。然而,我们对真菌如何对伤害和捕食者做出反应的了解很少。真菌像植物和动物一样对损伤做出反应,识别损伤和微生物相关分子模式(DAMPs/MAMPs),激活Ca2+和有丝分裂原激活的蛋白激酶依赖信号,激活防御机制。在食草过程中,植物除了激活与伤害相关的通路外,还激活特定的反应来对抗它们的捕食者。利用转录方法,我们研究了丝状真菌atroviridema对不同节肢动物的损伤和攻击激活特异性反应的能力。攻击黑胃果蝇抑制菌丝再生所需基因的转录激活,以及真菌的先天免疫和化学防御反应。我们还提供了涉及ded成分的这种抑制的机制见解。黑胃唾液腺,抑制一组基因的表达并阻止菌丝再生。
The capability to respond to wounding is a process shared by organisms of different kingdoms that can result in the regeneration of whole-body parts or lost structures or organs. Filamentous fungi constitute a rich food source that ensures survival and reproduction of their predators and are therefore continuously exposed to mechanical damage. Nevertheless, our understanding of how fungi respond to wounding and predators is scarce. Fungi like plants and animals respond to injury recognizing Damage- and Microbe-Associated Molecular Patterns (DAMPs/MAMPs) that activate Ca2+and Mitogen-Activated Protein Kinase dependent signaling for the activation of defense mechanisms. During herbivory, plants, in addition to activating pathways related to injury, activate specific responses to combat their predators. Using a transcriptional approach, we studied the capacity of the filamentous fungusTrichoderma atrovirideto activate specific responses to injury and attack by different arthropods. Attack byDrosophila melanogasterinhibited the transcriptional activation of genes required for hyphal regeneration, and the fungal innate immune and chemical defense responses. We also provide mechanistic insight of this inhibition involving components of theD. melanogastersalivary glands that repress the expression of a set of genes and block hyphal regeneration.
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