Mother's curse is pervasive across a large mitonuclear Drosophila panel.
Mother's curse is pervasive across a large mitonuclear Drosophila panel.
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DOI：
10.1002/evl3.221
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发表时间：
2021-06
影响因子：
5
通讯作者：
Camus MF
中科院分区：
文献类型：
--
作者：
Carnegie L;Reuter M;Fowler K;Lane N;Camus MF
线粒体基因组的母系遗传需要性别特异性的选择性筛，因此线粒体 DNA 的突变只能对作用于女性的选择做出反应。从理论上讲，这使得对男性有害的突变能够在线粒体基因组中积累，只要它们对女性是中性的、有益的或仅轻微有害的。最终，这种偏见可能会推动男性特异性线粒体突变负荷的进化，这种想法被称为“母亲的诅咒”。关于这一假设的早期工作主要使用小型果蝇组，其中天然来源的线粒体基因组与同基因核背景偶联。这些设计中缺乏核遗传变异，妨碍了稳健的泛化。在这里，我们使用大型果蝇线粒体核基因组测试了对母亲诅咒的预测，该基因组包含与九个线粒体单倍型耦合的九个等基因核基因组，总共提供了 81 种不同的线粒体核基因型。按照预测框架，我们通过筛选我们的小组的翅膀尺寸来测试母亲的诅咒假设。这一性状与整体体型密切相关，并且在果蝇中具有性别二态性。此外，生长在很大程度上依赖于新陈代谢和线粒体功能，这使得翅膀大小成为研究线粒体变异影响的理想特征。我们检测到高水平的线粒体核上位性，更重要的是，我们报告说，在使用的九个核遗传背景中，有八个果蝇的线粒体遗传变异在雄性果蝇中比雌性果蝇更大。这些结果表明，线粒体 DNA 的母系遗传确实以比之前证明的更普遍的方式调节男性生活史特征。
The maternal inheritance of mitochondrial genomes entails a sex‐specific selective sieve, whereby mutations in mitochondrial DNA can only respond to selection acting on females. In theory, this enables male‐harming mutations to accumulate in mitochondrial genomes as long as they are neutral, beneficial, or only slightly deleterious to females. Ultimately, this bias could drive the evolution of male‐specific mitochondrial mutation loads, an idea known as mother's curse. Earlier work on this hypothesis has mainly used small Drosophila panels, in which naturally sourced mitochondrial genomes were coupled to an isogenic nuclear background. The lack of nuclear genetic variation in these designs has precluded robust generalization. Here, we test the predictions of mother's curse using a large Drosophila mitonuclear genetic panel, comprising nine isogenic nuclear genomes coupled to nine mitochondrial haplotypes, giving a total of 81 different mitonuclear genotypes. Following a predictive framework, we tested the mother's curse hypothesis by screening our panel for wing size. This trait is tightly correlated with overall body size and is sexually dimorphic in Drosophila. Moreover, growth is heavily reliant on metabolism and mitochondrial function, making wing size an ideal trait for the study of the impact of mitochondrial variation. We detect high levels of mitonuclear epistasis, and more importantly, we report that mitochondrial genetic variance is larger in male than female Drosophila for eight out of the nine nuclear genetic backgrounds used. These results demonstrate that the maternal inheritance of mitochondrial DNA does indeed modulate male life history traits in a more generalisable way than previously demonstrated.
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DOI：
10.1098/rspb.2017.2257
发表时间：
2018-01-31
影响因子：
4.7
作者：
Connallon, Tim;Camus, M. Florencia;Dowling, Damian K.
通讯作者：
Dowling, Damian K.
影响因子：
5
作者：
Connallon, Tim;Matthews, Genevieve
通讯作者：
Matthews, Genevieve
影响因子：
9.2
作者：
Camus, M. Florencia;Clancy, David J.;Dowling, Damian K.
通讯作者：
Dowling, Damian K.
影响因子：
3.3
作者：
Camus, M. F.;Moore, J.;Reuter, M.
通讯作者：
Reuter, M.
影响因子：
16.8
作者：
Gemmell, NJ;Allendorf, FW
通讯作者：
Allendorf, FW