Bone morphogenetic protein-3b (BMP-3b) is expressed in adipocytes and inhibits adipogenesis as a unique complex.
Bone morphogenetic protein-3b (BMP-3b) is expressed in adipocytes and inhibits adipogenesis as a unique complex.
复制标题
DOI：
10.1038/ijo.2011.124
复制
发表时间：
2012-05
影响因子：
4.9
通讯作者：
Kangawa, K.
中科院分区：
文献类型：
--
作者：
Hino, J.;Miyazawa, T.;Miyazato, M.;Kangawa, K.
骨形态发生蛋白-3b（BMP-3b）是转化生长因子-β（TGF-β）超家族成员。BMP-3b调节骨生成，并在胚胎发育中发挥关键作用。BMP-3b不仅在骨和发育中的胚胎中表达，而且在脂肪组织中表达。然而，BMP-3b在脂肪组织中的功能仍然未知。BMP-3b的表达在各种脂肪组织和脂肪来源的基质-血管部分（SVF）和成熟脂肪细胞部分（AD.F）的小鼠进行定量。我们还使用3 T3-L1前脂肪细胞来分析BMP-3b的表达、功能和分子形式。为了确定BMP-3b对3 T3-L1细胞脂肪形成的影响，进行BMP-3b siRNA介导的敲低和基因过表达研究，并将含有BMP-3b蛋白的条件培养基（CM）加入3 T3-L1细胞培养物中。通过测量成脂标志物的表达或油红O染色来评估脂肪细胞分化。通过蛋白质印迹分析从3 T3-L1细胞分泌的BMP-3b的分子形式。BMP-3b在所有脂肪组织中表达，并且在前脂肪细胞中的表达水平高于成熟脂肪细胞。在肠系膜脂肪组织中，与对照小鼠相比，饮食诱导的肥胖（DIO）小鼠中BMP-3b的表达增加。BMP-3b在3 T3-L1细胞中也有高表达。我们发现siRNA介导的内源性BMP-3b表达的敲低在3 T3-L1细胞中增强了脂肪生成。相反，过度表达BMP-3b抑制脂肪细胞分化。我们还表明，添加含有BMP-3b蛋白的CM抑制3 T3-L1细胞的分化，并且通过用抗BMP-3b抗体去除BMP-3b来消除这种抑制作用。此外，BMP-3b作为独特的非共价复合物从脂肪细胞分泌。这些数据表明，BMP-3b是从脂肪细胞分泌，并参与脂肪细胞分化。
Bone morphogenetic protein-3b (BMP-3b) is a member of the transforming growth factor-β (TGF-β) superfamily. BMP-3b regulates osteogenesis and has critical roles in developing embryos. BMP-3b is expressed not only in the bone and developing embryos but also in adipose tissues. However, the functions of BMP-3b in adipose tissue are still unknown. BMP-3b expression was quantified in various adipose tissues and in the adipose-derived stromal-vascular fraction (SVF) and mature adipocyte fraction (AD.F) of mice. We also used 3T3-L1 preadipocytes to analyze the expression, function and molecular forms of BMP-3b. In order to determine the effects of BMP-3b on the adipogenesis of 3T3-L1 cells, BMP-3b siRNA-mediated knockdown and gene overexpression studies were performed, and a conditioned medium (CM) containing the BMP-3b protein was added to 3T3-L1 cell cultures. Adipocyte differentiation was evaluated by measuring the expression of adipogenic markers or by Oil Red O staining. The molecular form of BMP-3b that was secreted from the 3T3-L1 cells was analyzed by western blotting. BMP-3b is expressed in all adipose tissues and is expressed at higher levels in preadipocytes than in mature adipocytes. In mesenteric adipose tissue, BMP-3b expression was increased in diet-induced obesity (DIO) mice as compared with that in control mice. BMP-3b was also expressed highly in 3T3-L1 cells. We showed that siRNA-mediated knockdown of endogenous BMP-3b expression in 3T3-L1 cells enhanced adipogenesis. Conversely, overexpressing BMP-3b inhibited adipocyte differentiation. We also showed that addition of CM containing the BMP-3b protein inhibited the differentiation of 3T3-L1 cells, and that this inhibitory effect was abolished by removing BMP-3b with an anti-BMP-3b antibody. Furthermore, BMP-3b was secreted from adipocytes as a unique non-covalent complex. These data suggest that BMP-3b is secreted from adipocytes and is involved in adipocyte differentiation.
登录
查看更多内容
DOI：
10.1006/bbrc.1996.0889
发表时间：
1996-06-14
影响因子：
3.1
作者：
Hino, J;Takao, M;Kangawa, K
通讯作者：
Kangawa, K
DOI：
10.1006/bbrc.1999.0341
发表时间：
1999-03-16
影响因子：
3.1
作者：
Hino, J;Matsuo, H;Kangawa, K
通讯作者：
Kangawa, K
DOI：
10.1016/0006-291x(90)92081-a
发表时间：
1990-03-16
影响因子：
3.1
作者：
HINO, J;TATEYAMA, H;MATSUO, H
通讯作者：
MATSUO, H
DOI：
10.1006/bbrc.1996.0289
发表时间：
1996-02-15
影响因子：
3.1
作者：
Takao, M;Hino, J;Kangawa, K
通讯作者：
Kangawa, K
影响因子：
1.8
作者：
Chen, D;Zhao, M;Mundy, GR
通讯作者：
Mundy, GR