A Persistent Tuberculosis Outbreak in the UK Is Characterized by Hydrophobic fadB4-Deficient Mycobacterium tuberculosis That Replicates Rapidly in Macrophages.
A Persistent Tuberculosis Outbreak in the UK Is Characterized by Hydrophobic fadB4-Deficient Mycobacterium tuberculosis That Replicates Rapidly in Macrophages.
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DOI：
10.1128/mbio.02656-22
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发表时间：
2022-12-20
期刊：
影响因子：
6.4
通讯作者：
O'Hare HM
中科院分区：
文献类型：
--
作者：
Farzand R;Haigh RD;Monk P;Haldar P;Patel H;Pareek M;Verma R;Barer MR;Woltmann G;Ahyow L;Jagatia H;Decker J;Mukamolova GV;Cooper AM;Garton NJ;O'Hare HM
结核分枝杆菌的遗传多样性可以影响疾病的严重程度和传播性。为了更好地了解这种多样性如何影响个人和社区，我们对M进行了表型分析。结核病在英国东米德兰持续爆发。与非暴发菌株相比，芽孢杆菌具有更高的脂质含量和更疏水的细胞表面。在巨噬细胞感染模型中，细菌增加更快，引起巨噬细胞脂滴的增加和IL-1β的分泌增加。fadB 4、nrdB和plcC中的自然缺失将暴发分离株与该地区的其他谱系3分离株区分开来。fadB 4在细胞包膜生物合成中具有假定的作用，因此该基因的丢失有可能改变细菌与免疫细胞的相互作用。将fadB 4重新引入爆发菌株导致与非爆发菌株更相似的表型。对与暴发相关的微生物学特征和相应的遗传多态性的进一步了解有可能为结核病控制提供信息。
The genetic diversity of Mycobacterium tuberculosis can influence disease severity and transmissibility. To better understand how this diversity influences individuals and communities, we phenotyped M. tuberculosis that was causing a persistent outbreak in the East Midlands, United Kingdom. Compared to nonoutbreak isolates, bacilli had higher lipid contents and more hydrophobic cell surfaces. In macrophage infection models, the bacteria increased more rapidly, provoked the enhanced accumulation of macrophage lipid droplets and enhanced the secretion of IL-1β. Natural deletions in fadB4, nrdB, and plcC distinguished the outbreak isolates from other lineage 3 isolates in the region. fadB4 is annotated with a putative role in cell envelope biosynthesis, so the loss of this gene has the potential to alter the interactions of bacteria with immune cells. Reintroduction of fadB4 to the outbreak strain led to a phenotype that more closely resembled those of nonoutbreak strains. The improved understanding of the microbiological characteristics and the corresponding genetic polymorphisms that associate with outbreaks have the potential to inform tuberculosis control.
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影响因子：
14.9
作者：
Letunic I;Bork P
通讯作者：
Bork P
DOI：
10.1056/nejmoa1800474
发表时间：
2018-10-11
期刊：
The New England journal of medicine
影响因子：
--
作者：
CRyPTIC Consortium and the 100,000 Genomes Project;Allix-Béguec C;Arandjelovic I;Bi L;Beckert P;Bonnet M;Bradley P;Cabibbe AM;Cancino-Muñoz I;Caulfield MJ;Chaiprasert A;Cirillo DM;Clifton DA;Comas I;Crook DW;De Filippo MR;de Neeling H;Diel R;Drobniewski FA;Faksri K;Farhat MR;Fleming J;Fowler P;Fowler TA;Gao Q;Gardy J;Gascoyne-Binzi D;Gibertoni-Cruz AL;Gil-Brusola A;Golubchik T;Gonzalo X;Grandjean L;He G;Guthrie JL;Hoosdally S;Hunt M;Iqbal Z;Ismail N;Johnston J;Khanzada FM;Khor CC;Kohl TA;Kong C;Lipworth S;Liu Q;Maphalala G;Martinez E;Mathys V;Merker M;Miotto P;Mistry N;Moore DAJ;Murray M;Niemann S;Omar SV;Ong RT;Peto TEA;Posey JE;Prammananan T;Pym A;Rodrigues C;Rodrigues M;Rodwell T;Rossolini GM;Sánchez Padilla E;Schito M;Shen X;Shendure J;Sintchenko V;Sloutsky A;Smith EG;Snyder M;Soetaert K;Starks AM;Supply P;Suriyapol P;Tahseen S;Tang P;Teo YY;Thuong TNT;Thwaites G;Tortoli E;van Soolingen D;Walker AS;Walker TM;Wilcox M;Wilson DJ;Wyllie D;Yang Y;Zhang H;Zhao Y;Zhu B
通讯作者：
Zhu B
DOI：
10.1016/j.tube.2014.02.005
发表时间：
2014-05
期刊：
Tuberculosis (Edinburgh, Scotland)
影响因子：
--
作者：
Coll F;Preston M;Guerra-Assunção JA;Hill-Cawthorn G;Harris D;Perdigão J;Viveiros M;Portugal I;Drobniewski F;Gagneux S;Glynn JR;Pain A;Parkhill J;McNerney R;Martin N;Clark TG
通讯作者：
Clark TG
影响因子：
3.7
作者：
Bushnell B;Rood J;Singer E
通讯作者：
Singer E
影响因子：
11.8
作者：
Langlois-Klassen D;Senthilselvan A;Chui L;Kunimoto D;Saunders LD;Menzies D;Long R
通讯作者：
Long R