Curvature and Rho activation differentially control the alignment of cells and stress fibers.
Curvature and Rho activation differentially control the alignment of cells and stress fibers.
复制标题
DOI：
10.1126/sciadv.1700150
复制
发表时间：
2017-09
期刊：
影响因子：
13.6
通讯作者：
Stebe KJ
中科院分区：
文献类型：
--
作者：
Bade ND;Kamien RD;Assoian RK;Stebe KJ
Stress fibers, single cells, and densely packed cells align on gently curved surfaces and restructure upon Rho activation. In vivo, cells respond to a host of physical cues ranging from substrate stiffness to the organization of micro- and nanoscale fibrous networks. We show that macroscale substrates with radii of curvature from tens to hundreds of micrometers influence cell alignment. In a model system of fibroblasts, isolated cells aligned strongly in the axial direction on cylinders with radii similar to the cell length and more weakly on cylinders of much larger radius. Isolated vascular smooth muscle cells did not align as effectively as fibroblasts. However, both cell types aligned robustly in weak curvature fields when in confluent monolayers. We identified two distinct populations of stress fibers in both cell types: long, apical stress fibers that aligned axially and short, basal stress fibers that aligned circumferentially. Circumferential alignment of the basal stress fibers is in apparent disagreement with a long-standing hypothesis that energetic penalties for bending enforce axial alignment on cylinders. To explore this phenomenon, we manipulated stress fibers by activating Rho, a small guanosine triphosphatase that regulates stress fiber assembly. In response, apical stress fibers disassembled, whereas basal stress fibers thickened and aligned more strongly in the circumferential direction. By activating Rho in confluent monolayers of vascular smooth muscle cells, we recapitulated the circumferential alignment pattern of F-actin within these cells that is observed in cylindrical vessels in vivo. In agreement with recent theory, these results suggest that stress fiber bending penalties are overcome when stress fiber contractility is enhanced and motivate deeper study of the mechanics of these distinct stress fiber populations.
登录
查看更多内容
影响因子：
2.9
作者：
Amano, Mutsuki;Nakayama, Masanori;Kaibuchi, Kozo
通讯作者：
Kaibuchi, Kozo
影响因子：
3.7
作者：
DUNN, GA;HEATH, JP
通讯作者：
HEATH, JP
影响因子：
46.9
作者：
Kim, BS;Nikolovski, J;Mooney, DJ
通讯作者：
Mooney, DJ
循环应变频率在体外调节血管平滑肌细胞排列中的作用。
影响因子：
3.4
作者：
Liu, Bo;Qu, Ming-Juan;Jiang, Zong-Lai
通讯作者：
Jiang, Zong-Lai
DOI：
10.1115/1.2796072
发表时间：
1997-05-01
影响因子：
1.7
作者：
Barocas, VH;Tranquillo, RT
通讯作者：
Tranquillo, RT